Anti-GluA4 antibody [EPR2512(2)] (ab109431)
![Anti-GluA4 antibody [EPR2512(2)] (ab109431)](https://www.abcam.com/ps/products/109/ab109431/Images/ab109431-382386-anti-glua4-antibody-epr2512-2-westernblot-brain-rat.jpg "Anti-GluA4 antibody [EPR2512(2)] (ab109431)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2512(2)] to GluA4
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GluA4 antibody [EPR2512(2)]
See all GluA4 primary antibodies -
Description
Rabbit monoclonal [EPR2512(2)] to GluA4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Rat brain, Mouse brain, Human cerebellum, and SH-SY5Y lysates; SH-SY5Y cells
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General notes
Previously labelled as Ionotropic Glutamate receptor 4.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2512(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)All lanes : Anti-GluA4 antibody [EPR2512(2)] (ab109431) at 1/1000 dilution
Lane 1 : Rat brain prepared in RIPA lysis method
Lane 2 : Rat brain prepared in 1%SDS Hot lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 101 kDa
Observed band size: 101 kDaWe recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
Blocking/Diluting Buffer and concentration:
5% NFDM/TBST -
![Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)](https://www.abcam.com/ps/products/109/ab109431/Images/ab109431-122267-anti-glua4-antibody-epr25122-western-blot.jpg)
Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)All lanes : Anti-GluA4 antibody [EPR2512(2)] (ab109431) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Human cerebellum lysate
Lane 4 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 101 kDa
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![Flow Cytometry - Anti-GluA4 antibody [EPR2512(2)] (ab109431)](https://www.abcam.com/ps/products/109/ab109431/Images/ab109431-122266-anti-glua4-antibody-epr25122-flow-cytometry.jpg)
Flow Cytometry - Anti-GluA4 antibody [EPR2512(2)] (ab109431)Flow cytometric analysis of permeabilized SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells using ab109431 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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![Anti-GluA4 antibody [EPR2512(2)] (ab109431)](https://www.abcam.com/ps/products/109/ab109431/Images/ab109431-4-benefits-of-recombinant-antibodies.png)
Anti-GluA4 antibody [EPR2512(2)] (ab109431)
