Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GluA4 antibody [EPR2512(2)] (ab109431)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2512(2)] to GluA4
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GluA4 antibody [EPR2512(2)]
    See all GluA4 primary antibodies

  • Description

    Rabbit monoclonal [EPR2512(2)] to GluA4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Rat brain, Mouse brain, Human cerebellum, and SH-SY5Y lysates; SH-SY5Y cells

  • General notes

     

     

    Previously labelled as Ionotropic Glutamate receptor 4.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)
    Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)
    All lanes : Anti-GluA4 antibody [EPR2512(2)] (ab109431) at 1/1000 dilution

    Lane 1 : Rat brain prepared in RIPA lysis method
    Lane 2 : Rat brain prepared in 1%SDS Hot lysis method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 101 kDa
    Observed band size: 101 kDa



    We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

    Blocking/Diluting Buffer and concentration:
    5% NFDM/TBST

  • Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)
    Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)
    All lanes : Anti-GluA4 antibody [EPR2512(2)] (ab109431) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Human cerebellum lysate
    Lane 4 : SH-SY5Y lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 101 kDa

  • Flow Cytometry - Anti-GluA4 antibody [EPR2512(2)] (ab109431)
    Flow Cytometry - Anti-GluA4 antibody [EPR2512(2)] (ab109431)

    Flow cytometric analysis of permeabilized SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells using ab109431 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

  • Anti-GluA4 antibody [EPR2512(2)] (ab109431)
    Anti-GluA4 antibody [EPR2512(2)] (ab109431)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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