Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] - BSA and Azide free (ab247385)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2258IHCY] to EGFR (phospho Y1086) - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] - BSA and Azide free
    See all EGFR primary antibodies

  • Description

    Rabbit monoclonal [EP2258IHCY] to EGFR (phospho Y1086) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Recognises EGFR phosphorylated on Tyrosine 1086 of the mature human isoform 1 (corresponding to Y1110 from the precursor form P00533-1/p170).

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247385 is the carrier-free version of ab68470 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247385 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] - BSA and Azide free (ab247385)
    Western blot - Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] - BSA and Azide free (ab247385)
    All lanes : Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] (ab68470) at 1/5000 dilution

    Lane 1 : A431 cell lysate, untreated
    Lane 2 : A431 cell lysate, treated with EGF

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugatecd Goat anti-rabbit at 1/2000 dilution

    Predicted band size: 134 kDa
    Observed band size: 200 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab68470, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] - BSA and Azide free (ab247385)
    Immunocytochemistry - Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] - BSA and Azide free (ab247385)
    This data was developed using ab68470, the same antibody clone in a different buffer formulation.A431 cells stained with ab68470 at 1/100 dilution.
  • Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] - BSA and Azide free (ab247385)
    Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] - BSA and Azide free (ab247385)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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