Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] - BSA and Azide free (ab247385)
![Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] - BSA and Azide free (ab247385)](https://www.abcam.com/ps/products/247/ab247385/Images/ab247385-402314-anti-egfr-phospho-y1086-antibody-ep2258ihcy-bsa-and-azide-free-western-blot.jpg "Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] - BSA and Azide free (ab247385)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2258IHCY] to EGFR (phospho Y1086) - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] - BSA and Azide free
See all EGFR primary antibodies -
Description
Rabbit monoclonal [EP2258IHCY] to EGFR (phospho Y1086) - BSA and Azide free -
Host species
Rabbit -
Specificity
Recognises EGFR phosphorylated on Tyrosine 1086 of the mature human isoform 1 (corresponding to Y1110 from the precursor form P00533-1/p170).
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247385 is the carrier-free version of ab68470 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247385 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2258IHCY -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] - BSA and Azide free (ab247385)All lanes : Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] (ab68470) at 1/5000 dilution
Lane 1 : A431 cell lysate, untreated
Lane 2 : A431 cell lysate, treated with EGF
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugatecd Goat anti-rabbit at 1/2000 dilution
Predicted band size: 134 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?This data was developed using ab68470, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry - Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] - BSA and Azide free (ab247385)](https://www.abcam.com/ps/products/247/ab247385/Images/ab247385-1-anti-egfr-phospho-y1086-antibody-ep2258ihcy-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] - BSA and Azide free (ab247385) -
![Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] - BSA and Azide free (ab247385)](https://www.abcam.com/ps/products/247/ab247385/Images/ab247385-3-benefits-of-recombinant-antibodies.png)
Anti-EGFR (phospho Y1086) antibody [EP2258IHCY] - BSA and Azide free (ab247385)
