Anti-RAGE antibody [EPR12206] (ab172473)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12206] to RAGE
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-RAGE antibody [EPR12206]
See all RAGE primary antibodies -
Description
Rabbit monoclonal [EPR12206] to RAGE -
Host species
Rabbit -
Specificity
Recent tests in our laboratory showed that the antibody detects the band of interest in tissue lysates, but it did not detect the protein in cell lysate. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat -
Immunogen
Synthetic peptide within Human RAGE aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q15109 -
Positive control
- Mouse lung, Rat lung and Human placenta lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12206 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-RAGE antibody [EPR12206] (ab172473) at 1/1000 dilution
Lane 1 : Mouse lung lysates
Lane 2 : Mouse brain lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse heart lysates
Lane 5 : Mouse liver lysates
Lane 6 : Mouse spleen lysates
Lane 7 : Rat lung lysates
Lane 8 : Rat brain lysates with 5% NFDM/TBST
Lane 9 : Rat kidney lysates
Lane 10 : Rat heart lysates
Lane 11 : Rat spleen lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time:
Lane 1 to 6: 3 second
Lane 7 to 11: 20 secondsThe expression profile and molecular mass observed is consistent with what has been described in the literature (PMID: 16315007; 18355449; 18245812)
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All lanes : Anti-RAGE antibody [EPR12206] (ab172473) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Rat lung lysate
Lane 3 : Human placenta lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 42 kDa
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