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Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR826(2)] - BSA and Azide free (ab248499)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR826(2)] to Amyloid Precursor Protein (phospho T743) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR826(2)] - BSA and Azide free
    See all Amyloid Precursor Protein primary antibodies

  • Description

    Rabbit monoclonal [EPR826(2)] to Amyloid Precursor Protein (phospho T743) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects Amyloid beta precursor protein phosphorylated at Threonine 743.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    The immunogen used for this product is within Human Amyloid Precursor Protein aa 750 to the C-terminus and therefore may detect gamma secretase fragments 50, 57 and 59 in addition to fragments C31, C80, C83 and C99. Cross-reactivity with these fragments has not been confirmed experimentally.

    Ab248499 is the carrier-free version of ab133447. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248499 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR826(2)] - BSA and Azide free (ab248499)
    Western blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR826(2)] - BSA and Azide free (ab248499)
    All lanes : Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR826(2)] (ab133447) at 1/1000 dilution

    Lane 1 : Human brain lysate
    Lane 2 : Human brain lysate treated with lambda phosphatase.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 86 kDa



    This data was developed using ab133447, the same antibody clone in a different buffer formulation.

  • OI-RD Scanning - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR826(2)] - BSA and Azide free (ab248499)
    OI-RD Scanning - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR826(2)] - BSA and Azide free (ab248499)
    This data was developed using ab133447, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR826(2)] - BSA and Azide free (ab248499)
    Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR826(2)] - BSA and Azide free (ab248499)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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