Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP332Y] to SHC
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-SHC antibody [EP332Y]
See all SHC primary antibodies
Description

Rabbit monoclonal [EP332Y] to SHC
Host species

Rabbit
Specificity

This antibody detects p46, p52 and p66 -three isoforms of SHC.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Rat

Human

See all applications and species data
Immunogen

Synthetic peptide within Human SHC aa 500-600. The exact sequence is proprietary.
Positive control
- MCF7 whole cell lysate (ab3871) can be used as a positive control in WB. MCF 7 cell lysate
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP332Y
Isotype

IgG
Research areas

Western blot - Anti-SHC antibody [EP332Y] (ab33770)

All lanes : Anti-SHC antibody [EP332Y] (ab33770) at 1/1000 dilution

Lane 1 : C6 Cell lysate
Lane 2 : RAW264.7 Cell lysate
Lane 3 : NIH/3T3 Cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Predicted band size: 63 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC antibody [EP332Y] (ab33770)

ab33770 staining SHC in Human renal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/300). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC antibody [EP332Y] (ab33770)

ab33770 staining SHC in Human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/300). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
Western blot - Anti-SHC antibody [EP332Y] (ab33770)

All lanes : Anti-SHC antibody [EP332Y] (ab33770) at 1/1000 dilution

Lane 1 : MCF-7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Predicted band size: 63 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-SHC antibody [EP332Y] (ab33770)

Anti-SHC antibody [EP332Y] (ab33770) at 1/2000 dilution (unpurified) + MCF 7 cell lysate

Predicted band size: 63 kDa
Observed band size: 66 kDa
why is the actual band size different from the predicted?
Additional bands at: 46 kDa (possible isoform), 52 kDa (possible isoform)
Anti-SHC antibody [EP332Y] (ab33770)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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