Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SHC antibody [EP332Y] (ab33770)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP332Y] to SHC
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SHC antibody [EP332Y]
    See all SHC primary antibodies

  • Description

    Rabbit monoclonal [EP332Y] to SHC

  • Host species

    Rabbit

  • Specificity

    This antibody detects p46, p52 and p66 -three isoforms of SHC.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SHC aa 500-600. The exact sequence is proprietary.

  • Positive control

    • MCF7 whole cell lysate (ab3871) can be used as a positive control in WB. MCF 7 cell lysate

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-SHC antibody [EP332Y] (ab33770)
    Western blot - Anti-SHC antibody [EP332Y] (ab33770)
    All lanes : Anti-SHC antibody [EP332Y] (ab33770) at 1/1000 dilution

    Lane 1 : C6 Cell lysate
    Lane 2 : RAW264.7 Cell lysate
    Lane 3 : NIH/3T3 Cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

    Predicted band size: 63 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC antibody [EP332Y] (ab33770)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC antibody [EP332Y] (ab33770)

    ab33770 staining SHC in Human renal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/300). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC antibody [EP332Y] (ab33770)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC antibody [EP332Y] (ab33770)

    ab33770 staining SHC in Human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/300). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  • Western blot - Anti-SHC antibody [EP332Y] (ab33770)
    Western blot - Anti-SHC antibody [EP332Y] (ab33770)
    All lanes : Anti-SHC antibody [EP332Y] (ab33770) at 1/1000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

    Predicted band size: 63 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-SHC antibody [EP332Y] (ab33770)
    Western blot - Anti-SHC antibody [EP332Y] (ab33770)
    Anti-SHC antibody [EP332Y] (ab33770) at 1/2000 dilution (unpurified) + MCF 7 cell lysate

    Predicted band size: 63 kDa
    Observed band size: 66 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 46 kDa (possible isoform), 52 kDa (possible isoform)

  • Anti-SHC antibody [EP332Y] (ab33770)
    Anti-SHC antibody [EP332Y] (ab33770)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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