Anti-SHC (phospho Y239 + Y240) antibody [EP419] (ab109455)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP419] to SHC (phospho Y239 + Y240)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-SHC (phospho Y239 + Y240) antibody [EP419]
See all SHC primary antibodies -
Description
Rabbit monoclonal [EP419] to SHC (phospho Y239 + Y240) -
Host species
Rabbit -
Specificity
ab109455 only detects SHC phosphorylated at Tyrosine 239/240. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T cell lysates, treated with EGF.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP419 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-SHC (phospho Y239 + Y240) antibody [EP419] (ab109455) at 1/5000 dilution
Lane 1 : 293T cell lysates, EGF treated with single phospho (pY239) peptide
Lane 2 : 293T cell lysates, EGF treated with single phospho (pY240) peptide
Lane 3 : 293T cell lysates, EGF treated with Double phospho (pY239/pY240) peptide
Lane 4 : 293T cell lysates, EGF treated with non-phospho peptide blocking
Lane 5 : 293T cell lysates, EGF treated
Lysates/proteins at 10 µg per lane.
Predicted band size: 63 kDa
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All lanes : Anti-SHC (phospho Y239 + Y240) antibody [EP419] (ab109455) at 1/500 dilution
Lane 1 : 293T cell lysates, untreated
Lane 2 : 293T cell lysates, treated with EGF
Lysates/proteins at 10 µg per lane.
Predicted band size: 63 kDa
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