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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Amyloid

Anti-SHC antibody (ab131515)

Anti-SHC antibody (ab131515)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to SHC
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SHC antibody
    See all SHC primary antibodies
  • Description

    Rabbit polyclonal to SHC
  • Host species

    Rabbit
  • Specificity

    ab131515 detects endogenous levels of total SHC protein.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH, surrounding amino acids 425-429 (P-S-Y-V-N) of Human SHC (NP_001123512.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa cells; 293 cell extract; Human breast carcinoma tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab131515 was purified by affinity chromatography using epitope specific peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Amyloid
    • Signal Transduction
    • Adapters
    • Cytoplasmic

Images

  • Western blot - Anti-SHC antibody (ab131515)
    Western blot - Anti-SHC antibody (ab131515)
    All lanes : Anti-SHC antibody (ab131515) at 1/500 dilution

    Lane 1 : 293 cell extract with blocking peptide
    Lane 2 : 293 cell extract

    Predicted band size: 62 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SHC antibody (ab131515)
    Immunocytochemistry/ Immunofluorescence - Anti-SHC antibody (ab131515)
    Immunofluorescent analysis of methanol-fixed HeLa cells labelling SHC with ab131515 at 1/100 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC antibody (ab131515)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHC antibody (ab131515)
    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling SHC with ab131515 at 1/50 dilution. The image on the right is treated with the synthesized peptide.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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