Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-USP11 antibody [EPR4346] - BSA and Azide free (ab239957)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4346] to USP11 - BSA and Azide free
  • Suitable for: Flow Cyt, IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-USP11 antibody [EPR4346] - BSA and Azide free
    See all USP11 primary antibodies

  • Description

    Rabbit monoclonal [EPR4346] to USP11 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab239957 is the carrier-free version of ab109232. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239957 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-USP11 antibody [EPR4346] - BSA and Azide free (ab239957)
    Immunoprecipitation - Anti-USP11 antibody [EPR4346] - BSA and Azide free (ab239957)
    ab109232 (purified) at 1/300 immunoprecipitating USP11 in 10 µg HEK293 (Lanes 1 and 2, observed at 110 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109232).

  • Immunoprecipitation - Anti-USP11 antibody [EPR4346] - BSA and Azide free (ab239957)
    Immunoprecipitation - Anti-USP11 antibody [EPR4346] - BSA and Azide free (ab239957)
    ab109232 (purified) at 1/300 immunoprecipitating USP11 in 10 µg Jurkat (Lanes 1 and 2, observed at 110 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109232).

  • Flow Cytometry - Anti-USP11 antibody [EPR4346] - BSA and Azide free (ab239957)
    Flow Cytometry - Anti-USP11 antibody [EPR4346] - BSA and Azide free (ab239957)
    Overlay histogram showing Jurkat cells fixed in 4% PFA and stained with purified ab109232 at a dilution of 1 in 950 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109232).

  • Flow Cytometry - Anti-USP11 antibody [EPR4346] - BSA and Azide free (ab239957)
    Flow Cytometry - Anti-USP11 antibody [EPR4346] - BSA and Azide free (ab239957)

    Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling USP11 with unpurified ab109232 at 1/570 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line). The unlabeled control is cells without incubation with primary and secondary antibodies (blue line).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109232).

  • Anti-USP11 antibody [EPR4346] - BSA and Azide free (ab239957)
    Anti-USP11 antibody [EPR4346] - BSA and Azide free (ab239957)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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