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Anti-GRP78 BiP antibody (ab227865)

Anti-GRP78 BiP antibody (ab227865)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to GRP78 BiP
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-GRP78 BiP antibody
    See all GRP78 BiP primary antibodies
  • Description

    Rabbit polyclonal to GRP78 BiP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Xenopus laevis, Zebrafish, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human GRP78 BiP (internal sequence). The exact sequence is proprietary.
    Database link: P11021

  • Positive control

    • WB: HepG2, HEK-293T, A431, HeLa, NIH/3T3, JC, BCL-1, PC-12 and Rat2 whole cell extracts. ICC/IF: A431 cells. IHC-P: Mouse and rat brain tissues
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • ER

Images

  • Western blot - Anti-GRP78 BiP antibody (ab227865)
    Western blot - Anti-GRP78 BiP antibody (ab227865)
    All lanes : Anti-GRP78 BiP antibody (ab227865) at 1/10000 dilution

    Lane 1 : Non-transfected HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
    Lane 2 : GRP78 BiP shRNA transfected HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 72 kDa



    7.5% SDS PAGE gel.

  • Immunocytochemistry/ Immunofluorescence - Anti-GRP78 BiP antibody (ab227865)
    Immunocytochemistry/ Immunofluorescence - Anti-GRP78 BiP antibody (ab227865)

    4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for GRP78 BiP (green) using ab227865 at 1/500 dilution in ICC/IF.

    Nuclear counter stain: Hoechst 33342 (blue).

  • Western blot - Anti-GRP78 BiP antibody (ab227865)
    Western blot - Anti-GRP78 BiP antibody (ab227865)
    All lanes : Anti-GRP78 BiP antibody (ab227865) at 1/10000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 72 kDa



    7.5% SDS PAGE gel.

  • Western blot - Anti-GRP78 BiP antibody (ab227865)
    Western blot - Anti-GRP78 BiP antibody (ab227865)
    All lanes : Anti-GRP78 BiP antibody (ab227865) at 1/10000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma) whole cell extract
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 72 kDa



    7.5% SDS PAGE gel.

  • Western blot - Anti-GRP78 BiP antibody (ab227865)
    Western blot - Anti-GRP78 BiP antibody (ab227865)
    All lanes : Anti-GRP78 BiP antibody (ab227865) at 1/10000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL-1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 72 kDa



    7.5% SDS PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody (ab227865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody (ab227865)

    Paraffin-embedded mouse brain tissue stained for GRP78 BiP using ab227865 at 1/250 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody (ab227865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody (ab227865)

    Paraffin-embedded rat brain tissue stained for GRP78 BiP using ab227865 at 1/250 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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