Anti-GRP78 BiP antibody (ab227865)
Key features and details
- Rabbit polyclonal to GRP78 BiP
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GRP78 BiP antibody
See all GRP78 BiP primary antibodies -
Description
Rabbit polyclonal to GRP78 BiP -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Xenopus laevis, Zebrafish, Rhesus monkey -
Immunogen
Recombinant fragment within Human GRP78 BiP (internal sequence). The exact sequence is proprietary.
Database link: P11021 -
Positive control
- WB: HepG2, HEK-293T, A431, HeLa, NIH/3T3, JC, BCL-1, PC-12 and Rat2 whole cell extracts. ICC/IF: A431 cells. IHC-P: Mouse and rat brain tissues
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-GRP78 BiP antibody (ab227865) at 1/10000 dilution
Lane 1 : Non-transfected HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 2 : GRP78 BiP shRNA transfected HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 72 kDa7.5% SDS PAGE gel.
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4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for GRP78 BiP (green) using ab227865 at 1/500 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).
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All lanes : Anti-GRP78 BiP antibody (ab227865) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 72 kDa7.5% SDS PAGE gel.
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All lanes : Anti-GRP78 BiP antibody (ab227865) at 1/10000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma) whole cell extract
Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 72 kDa7.5% SDS PAGE gel.
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All lanes : Anti-GRP78 BiP antibody (ab227865) at 1/10000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 72 kDa7.5% SDS PAGE gel.
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Paraffin-embedded mouse brain tissue stained for GRP78 BiP using ab227865 at 1/250 dilution in immunohistochemical analysis.
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Paraffin-embedded rat brain tissue stained for GRP78 BiP using ab227865 at 1/250 dilution in immunohistochemical analysis.