Recombinant human EZH1 + EED + SUZ12 + RBBP4 + AEBP2 protein (Active) (ab196413)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 79% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, DDDDK tag N-Terminus, His tag N-Terminus, His tag N-Terminus, His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human EZH1 + EED + SUZ12 + RBBP4 + AEBP2 protein (Active) -
Biological activity
50 µl reaction mix (20 mM phosphate pH 7.4, 0.05% Tween-20, 40 µM S-adenosylmethionine, and ab196413 were added to the wells coated with the substrate on a Neutravidin white plate. Incubated for 1 hr. Added antibody against methylated K27 residue of histone H3, incubated 1 hr. Then, added secondary HRP-labeled antibody and incubated 30 min. Finally, added HRP chemiluminescent substrates and read luminescence.
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Purity
>= 79 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
EIPNPPTSKCITYWKRKVKSEYMRLRQLKRLQANMGAKALYVANFAKVQE KTQILNEEWKKLRVQPVQSMKPVSGHPFLKKCTIESIFPGFASQHMLMRS LNTVALVPIMYSWSPLQQNFMVEDETVLCNIPYMGDEVKEEDETFIEELI NNYDGKVHGEEEMIPGSVLISDAVFLELVDALNQYSDEEEEGHNDTSDGK QDDSKEDLPVTRKRKRHAIEGNKKSSKKQFPNDMIFSAIASMFPENGVPD DMKERYRELTEMSDPNALPPQCTPNIDGPNAKSVQREQSLHSFHTLFCRR CFKYDCFLHPFHATPNVYKRKNKEIKIEPEPCGTDCFLLLEGAKEYAMLH NPRSKCSGRRRRRHHIVSASCSNASASAVAETKEGDSDRDTGNDWASSSS EANSRCQTPTKQKASPAPPQLCVVEAPSEPVEWTGAEESLFRVFHGTYFN NFCSIARLLGTKTCKQVFQFAVKESLILKLPTDELMNPSQKKKRKHRLWA AHCRKIQLKKDNSSTQVYNYQPCDHPDRPCDSTCPCIMTQNFCEKFCQCN PDCQNRFPGCRCKTQCNTKQCPCYLAVRECDPDLCLTCGASEHWDCKVVS CKNCSIQRGLKKHLLLAPSDVAGWGTFIKESVQKNEFISEYCGELISQDE ADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVV MVNGDHRIGIFAKRAIQAGEELFFDYRYSQADALKYVGIERETDVL -
Molecular weight information
86 kDa by SDS-PAGE -
Amino acids
2 to 747 -
Tags
His tag N-Terminus -
Additional sequence information
EZH1; NM_001991.
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Amino Acid Sequence 2
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Species
Human -
Sequence
SEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTE RPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQF NWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCA WTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPR DPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKI MSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFST RDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVT ILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKC TTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR -
Molecular weight information
51 kDa by SDS-PAGE -
Amino acids
2 to 441 -
Tags
DDDDK tag N-Terminus -
Additional sequence information
EED: NM_003797.
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Amino Acid Sequence 3
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Species
Human -
Sequence
APQKHGGGGGGGSGPSAGSGGGGFGGSAAVAAATASGGKSGGGSCGGGGS YSASSSSSAAAAAGAAVLPVKKPKMEHVQADHELFLQAFEKPTQIYRFLR TRNLIAPIFLHRTLTYMSHRNSRTNIKRKTFKVDDMLSKVEKMKGEQESH SLSAHLQLTFTGFFHKNDKPSPNSENEQNSVTLEVLLVKVCHKKRKDVSC PIRQVPTGKKQVPLNPDLNQTKPGNFPSLAVSSNEFEPSNSHMVKSYSLL FRVTRPGRREFNGMINGETNENIDVNEELPARRKRNREDGEKTFVAQMTV FDKNRRLQLLDGEYEVAMQEMEECPISKKRATWETILDGKRLPPFETFSQ GPTLQFTLRWTGETNDKSTAPIAKPLATRNSESLHQENKPGSVKPTQTIA VKESLTTDLQTRKEKDTPNENRQKLRIFYQFLYNNNTRQQTEARDDLHCP WCTLNCRKLYSLLKHLKLCHSRFIFNYVYHPKGARIDVSINECYDGSYAG NPQDIHRQPGFAFSRNGPVKRTPITHILVCRPKRTKASMSEFLESEDGEV EQQRTYSSGHNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIE EFSDVNEGEKEVMKLWNLHVMKHGFIADNQMNHACMLFVENYGQKIIKKN LCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQQKLEKGESASPANEEI TEEQNGTANGFSEINSKEKALETDSVSGVSKQSKKQKL -
Molecular weight information
87 kDa by SDS-PAGE -
Amino acids
2 to 739 -
Tags
His tag N-Terminus -
Additional sequence information
SUZ12: NM_015355.
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Amino Acid Sequence 4
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Species
Human -
Sequence
AAAITDMADLEELSRLSPLPPGSPGSAARGRAEPPEEEEEEEEEEEEAEA EAVAALLLNGGSGGGGGGGGGGVGGGEAETMSEPSPESASQAGEDEDEEE DDEEEEDESSSSGGGEEESSAESLVGSSGGSSSDETRSLSPGAASSSSGD GDGKEGLEEPKGPRGSQGGGGGGSSSSSVVSSGGDEGYGTGGGGSSATSG GRRGSLEMSSDGEPLSRMDSEDSISSTIMDVDSTISSGRSTPAMMNGQGS TTSSSKNIAYNCCWDQCQACFNSSPDLADHIRSIHVDGQRGGVFVCLWKG CKVYNTPSTSQSWLQRHMLTHSGDKPFKCVVGGCNASFASQGGLARHVPT HFSQQNSSKVSSQPKAKEESPSKAGMNKRRKLKNKRRRSLPRPHDFFDAQ TLDAIRHRAICFNLSAHIESLGKGHSVVFHSTVIAKRKEDSGKIKLLLHW MPEDILPDVWVNESERHQLKTKVVHLSKLPKDTALLLDPNIYRTMPQKRL KR -
Molecular weight information
53 kDa by SDS-PAGE -
Amino acids
2 to 503 -
Tags
His tag N-Terminus -
Additional sequence information
AEBP2: NM_153207.
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Amino Acid Sequence 5
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Species
Human -
Sequence
ADKEAAFDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLP DVTRPEGKDFSIHRLVLGTHTSDEQNHLVIASVQLPNDDAQFDASHYDSE KGEFGGFGSVSGKIEIEIKINHEGEVNRARYMPQNPCIIATKTPSSDVLV FDYTKHPSKPDPSGECNPDLRLRGHQKEGYGLSWNPNLSGHLLSASDDHT ICLWDISAVPKEGKVVDAKTIFTGHTAVVEDVSWHLLHESLFGSVADDQK LMIWDTRSNNTSKPSHSVDAHTAEVNCLSFNPYSEFILATGSADKTVALW DLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLNVWDLSKIG EEQSPEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQV WQMAENIYNDEDPEGSVDPEGQGS -
Molecular weight information
48 kDa by SDS-PAGE -
Amino acids
2 to 425 -
Tags
His tag N-Terminus -
Additional sequence information
RbAp48: NM_005610.
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Preparation and Storage
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Amino Acid Sequence 5
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Species
Human -
Sequence
ADKEAAFDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLP DVTRPEGKDFSIHRLVLGTHTSDEQNHLVIASVQLPNDDAQFDASHYDSE KGEFGGFGSVSGKIEIEIKINHEGEVNRARYMPQNPCIIATKTPSSDVLV FDYTKHPSKPDPSGECNPDLRLRGHQKEGYGLSWNPNLSGHLLSASDDHT ICLWDISAVPKEGKVVDAKTIFTGHTAVVEDVSWHLLHESLFGSVADDQK LMIWDTRSNNTSKPSHSVDAHTAEVNCLSFNPYSEFILATGSADKTVALW DLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLNVWDLSKIG EEQSPEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQV WQMAENIYNDEDPEGSVDPEGQGS -
Molecular weight information
48 kDa by SDS-PAGE -
Amino acids
2 to 425 -
Tags
His tag N-Terminus -
Additional sequence information
RbAp48: NM_005610.
Images
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Functional Studies - Recombinant human EZH1 + EED + SUZ12 + RBBP4 + AEBP2 protein (Active) (ab196413)Specific activity of ab196413.
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SDS-PAGE - Recombinant human EZH1 + EED + SUZ12 + RBBP4 + AEBP2 protein (Active) (ab196413)SDS-PAGE analysis of ab196413.