Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ADAM9 antibody [EPR21924-164] - BSA and Azide free (ab238647)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21924-164] to ADAM9 - BSA and Azide free
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ADAM9 antibody [EPR21924-164] - BSA and Azide free
    See all ADAM9 primary antibodies

  • Description

    Rabbit monoclonal [EPR21924-164] to ADAM9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, HeLa, WI-38, MCF7, U-87 MG and A549 whole cell lysate.

  • General notes

    Ab238647 is the carrier-free version of ab218242. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238647 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21924-164

  • Isotype

    IgG

Images

  • Western blot - Anti-ADAM9 antibody [EPR21924-164] - BSA and Azide free (ab238647)
    Western blot - Anti-ADAM9 antibody [EPR21924-164] - BSA and Azide free (ab238647)
    All lanes : Anti-ADAM9 antibody [EPR21924-164] (ab218242) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ADAM9 knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution

    Predicted band size: 91 kDa
    Observed band size: 100,75 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab218242).

    Lanes 1-3: Merged signal (red and green). Green - ab218242 observed at 100,75 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab218242 Anti-ADAM9 antibody [EPR21924-164] was shown to specifically react with ADAM9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265484 (knockout cell lysate ab257814) was used. Wild-type and ADAM9 knockout samples were subjected to SDS-PAGE. ab218242 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunoprecipitation - Anti-ADAM9 antibody [EPR21924-164] - BSA and Azide free (ab238647)
    Immunoprecipitation - Anti-ADAM9 antibody [EPR21924-164] - BSA and Azide free (ab238647)

    ADAM9 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma cell) whole cell lysate with ab218242 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218242 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10μg (input).
    Lane 2: ab218242 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218242 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    The conformational epitope maybe changed after cleavage and this antibody has better affinity to the pro-form.

    The molecular mass observed is consistent with what has been described in the literature (PMID 29118335; PMID: 12767059; PMID: 20736367).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218242).

  • Western blot - Anti-ADAM9 antibody [EPR21924-164] - BSA and Azide free (ab238647)
    Western blot - Anti-ADAM9 antibody [EPR21924-164] - BSA and Azide free (ab238647)
    All lanes : Anti-ADAM9 antibody [EPR21924-164] (ab218242) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : WI-38 (human fetal lung fibroblast), whole cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 5 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
    Lane 6 : A549 (human lung carcinoma epithelial cell), whole cell lysate
    Lane 7 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 91 kDa



    Blocking/diluting buffer and concentration:  5% NFDM/TBST 

    Exposure times:
    Lanes 1-5: 3 minutes
    Lane 6: 48 seconds
    Lane 7: 114 seconds

    The molecular mass observed is consistent with what has been described in the literature (PMID 29118335; PMID: 12767059; PMID: 20736367)

    We recommend that customers avoid boiling their samples to prevent protein aggregation.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218242).

  • Anti-ADAM9 antibody [EPR21924-164] - BSA and Azide free (ab238647)
    Anti-ADAM9 antibody [EPR21924-164] - BSA and Azide free (ab238647)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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