Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸ 10 µl 117 264 ₸

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Anti-ADAM9 antibody [EPR21924-164] (ab218242)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21924-164] to ADAM9
Suitable for: IP, WB
Knockout validated
Reacts with: Human

Product name

Anti-ADAM9 antibody [EPR21924-164]
See all ADAM9 primary antibodies
Description

Rabbit monoclonal [EPR21924-164] to ADAM9
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HepG2, HeLa, WI-38, MCF7, U-87 MG and A549 whole cell lysates. IP: HeLa whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21924-164
Isotype

IgG

Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)

All lanes : Anti-ADAM9 antibody [EPR21924-164] (ab218242) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ADAM9 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 91 kDa
Observed band size: 100,75 kDa
why is the actual band size different from the predicted?

Lanes 1-3: Merged signal (red and green). Green - ab218242 observed at 100,75 kDa. Red - loading control ab8245 observed at 36 kDa.

ab218242 Anti-ADAM9 antibody [EPR21924-164] was shown to specifically react with ADAM9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265484 (knockout cell lysate ab257814) was used. Wild-type and ADAM9 knockout samples were subjected to SDS-PAGE. ab218242 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)

All lanes : Anti-ADAM9 antibody [EPR21924-164] (ab218242) at 1/1000 dilution

Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : WI-38 (human fetal lung fibroblast), whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 5 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
Lane 6 : A549 (human lung carcinoma epithelial cell), whole cell lysate
Lane 7 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 91 kDa
Observed band size: 100,75 kDa why is the actual band size different from the predicted?

Blocking/diluting buffer and concentration: 5% NFDM/TBST

Exposure times:
Lanes 1-5: 3 minutes
Lane 6: 48 seconds
Lane 7: 114 seconds

The molecular mass observed is consistent with what has been described in the literature (PMID 29118335; PMID: 12767059; PMID: 20736367).

We recommend that customers avoid boiling their samples to prevent protein aggregation.
Immunoprecipitation - Anti-ADAM9 antibody [EPR21924-164] (ab218242)

ADAM9 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma cell) whole cell lysate with ab218242 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218242 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

Lane 1: HeLa whole cell lysate 10μg (input).
Lane 2: ab218242 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218242 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.

The conformational epitope maybe changed after cleavage and this antibody has better affinity to the pro-form.

The molecular mass observed is consistent with what has been described in the literature (PMID 29118335; PMID: 12767059; PMID: 20736367).
Anti-ADAM9 antibody [EPR21924-164] (ab218242)

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