Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free (ab280960)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24151-69] to GNAO1 - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free
    See all GNAO1 primary antibodies

  • Description

    Rabbit monoclonal [EPR24151-69] to GNAO1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human cerebellum, IMR-32, Neuro-2a, NIH/3T3, Mouse brain, cerebellum, hippocampus and rat brain lysates. IHC-P: Human cerebellum, mouse cerebellum, rat cerebellum tissues. IHC-Fr: mouse cerebellum, rat cerebellum tissues.

  • General notes

    ab280960 is the carrier-free version of ab259884. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280960 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free (ab280960)
    Western blot - Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free (ab280960)
    Anti-GNAO1 antibody [EPR24151-69] (ab259884) at 1/1000 dilution + Human cerebellum tissue lysate at 10 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 40 kDa



    This data was developed using ab259884, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 5.5 seconds

     

  • Western blot - Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free (ab280960)
    Western blot - Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free (ab280960)
    All lanes : Anti-GNAO1 antibody [EPR24151-69] (ab259884) at 1/1000 dilution

    Lane 1 : IMR-32 (human neuroblastoma neuroblast) whole cell lysate
    Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 40 kDa



    This data was developed using ab259884, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure time: 3 minutes

     

  • Western blot - Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free (ab280960)
    Western blot - Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free (ab280960)
    All lanes : Anti-GNAO1 antibody [EPR24151-69] (ab259884) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse cerebellum tissue lysate
    Lane 3 : Mouse hippocampus tissue lysate
    Lane 4 : Mouse heart tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 40 kDa



    This data was developed using ab259884, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This blot was developed using a higher sensitivity ECL.

    Samples are non-boiled as boiling may cause protein aggregates.

     

    The MW observed is consistent with the literature (PMID: 14519434).

     Exposure time: 7.75 seconds

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free (ab280960)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free (ab280960)

    This data was developed using ab259884, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labelling GNAO1 with ab259884 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebellum. The section was incubated with ab259884 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free (ab280960)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free (ab280960)

    This data was developed using ab259884, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labelling GNAO1 with ab259884 at 1/5000 (0.104 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebellum. The section was incubated with ab259884 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free (ab280960)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free (ab280960)

    This data was developed using ab259884, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labelling GNAO1 with ab259884 at 1/5000 (0.104 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebellum. The section was incubated with ab259884 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free (ab280960)
    Immunohistochemistry (Frozen sections) - Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free (ab280960)

    This data was developed using ab259884, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum tissue labeling GNAO1 with ab259884 at 1/100 (5.22 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free (ab280960)
    Immunohistochemistry (Frozen sections) - Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free (ab280960)

    This data was developed using ab259884, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebellum tissue labeling GNAO1 with ab259884 at 1/100 (5.22 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat cerebellum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free (ab280960)
    Anti-GNAO1 antibody [EPR24151-69] - BSA and Azide free (ab280960)

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