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Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free (ab247438)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1326-80-5] to Cytochrome C - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free
    See all Cytochrome C primary antibodies

  • Description

    Rabbit monoclonal [EP1326-80-5] to Cytochrome C - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247438 is the carrier-free version of ab76107. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247438 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free (ab247438)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free (ab247438)
    This data was developed using ab76107, the same antibody clone in a different buffer formulation.ab76107 at 1/100 dilution staining Cytochrome C in human kidney by Immunohistochemistry using paraffin-embedded tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free (ab247438)
    Western blot - Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free (ab247438)
    All lanes : Anti-Cytochrome C antibody [EP1326-80-5] (ab76107) at 1/200 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : COS cell lysate
    Lane 3 : L929 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-2 : Goat anti-rabbit HRP at 1/2000 dilution
    Lane 3 : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 12 kDa
    Observed band size: 15 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab76107, the same antibody clone in a different buffer formulation.

     

  • Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free (ab247438)
    Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free (ab247438)

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