Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free (ab247438)
![Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free (ab247438)](https://www.abcam.com/ps/products/247/ab247438/Images/ab247438-2-anti-cytochrome-c-antibody-ep1326-80-5-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif "Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free (ab247438)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1326-80-5] to Cytochrome C - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free
See all Cytochrome C primary antibodies -
Description
Rabbit monoclonal [EP1326-80-5] to Cytochrome C - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247438 is the carrier-free version of ab76107. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247438 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP1326-80-5 -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free (ab247438)This data was developed using ab76107, the same antibody clone in a different buffer formulation.ab76107 at 1/100 dilution staining Cytochrome C in human kidney by Immunohistochemistry using paraffin-embedded tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free (ab247438)](https://www.abcam.com/ps/products/247/ab247438/Images/ab247438-397942-anti-cytochrome-c-antibody-ep1326-80-5-bsa-and-azide-free-western-blot.gif)
Western blot - Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free (ab247438)All lanes : Anti-Cytochrome C antibody [EP1326-80-5] (ab76107) at 1/200 dilution
Lane 1 : HeLa cell lysate
Lane 2 : COS cell lysate
Lane 3 : L929 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Goat anti-rabbit HRP at 1/2000 dilution
Lane 3 : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 12 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?This data was developed using ab76107, the same antibody clone in a different buffer formulation.
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![Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free (ab247438)](https://www.abcam.com/ps/products/247/ab247438/Images/ab247438-3-benefits-of-recombinant-antibodies.png)
Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free (ab247438)
