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Recombinant Human PEDF protein (ab152076)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE, HPLC

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 0.88% Sodium chloride

  • Reconstitution
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at

    General Info

    • Alternative names

      • Cell proliferation-inducing gene 35 protein
      • EPC 1
      • EPC-1
      • EPC1
      • OI12
      • OI6
      • PEDF
      • PEDF_HUMAN
      • PIG 35
      • PIG35
      • Pigment epithelium derived factor
      • Pigment epithelium-derived factor
      • Proliferation inducing protein 35
      • Serine (or cysteine) proteinase inhibitor
      • serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
      • Serpin F1
      • Serpin family F member 1
      • Serpin peptidase inhibitor
      • Serpin peptidase inhibitor clade F member 1
      • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
      • SERPINF 1
      • Serpinf1
      see all

    • Function

      Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

    • Tissue specificity

      Retinal pigment epithelial cells and blood plasma.

    • Sequence similarities

      Belongs to the serpin family.

    • Developmental stage

      Expressed in quiescent cells.

    • Domain

      The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.

    • Post-translational
      modifications

      The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
      N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

    • Cellular localization

      Secreted. Melanosome. Enriched in stage I melanosomes.
    • Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

References (0)

Publishing research using ab152076? Please let us know so that we can cite the reference in this datasheet.

ab152076 has not yet been referenced specifically in any publications.

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