Anti-FOXP1 antibody (ab16645)
Key features and details
- Rabbit polyclonal to FOXP1
- Suitable for: IHC-FoFr, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-FOXP1 antibody
See all FOXP1 primary antibodies -
Description
Rabbit polyclonal to FOXP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-FoFr MouseWB Mouse
-
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human FOXP1.
Read Abcam's proprietary immunogen policy (Peptide available as ab20276.) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-FoFr MouseWB MouseAll applications RatChickenDogHumanXenopus laevisApplication Abreviews Notes IHC-FoFr Use at an assay dependent concentration.WB (6) 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).ab16645 recognises the different isoforms of FOXP1 in Jurkat cell lysate (see review for further details).
Notes IHC-FoFr
Use at an assay dependent concentration.WB
1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).ab16645 recognises the different isoforms of FOXP1 in Jurkat cell lysate (see review for further details).
Target
-
Function
Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development. -
Involvement in disease
Note=A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5.
Defects in FOXP1 are the cause of mental retardation with language impairment and autistic features (MRLIAF) [MIM:613670]. It is a developmental disorder characterized by mild to moderate mental retardation, language impairment, and autistic features. Patients show global delay, delayed walking, severely delayed speech development, and behavioral abnormalities, including irritability, hyperactivity, aggression, and stereotypical rigid behaviors. -
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
Domain
The leucine-zipper is required for dimerization and transcriptional repression. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 416092 Chicken
- Entrez Gene: 484692 Dog
- Entrez Gene: 27086 Human
- Entrez Gene: 108655 Mouse
- Entrez Gene: 297480 Rat
- Entrez Gene: 496386 Xenopus laevis
- Entrez Gene: 779033 Xenopus laevis
- Omim: 605515 Human
see all -
Alternative names
- 12CC4 antibody
- FLJ23741 antibody
- Fork head related protein like B antibody
see all
Images
-
Western blot - Anti-FOXP1 antibody (ab16645)All lanes : Anti-FOXP1 antibody (ab16645) at 1/5000 dilution
Lane 1 : Lysate from e12.5 whole mouse embryo FoxP1 +/-
Lane 2 : Lysate from e12.5 whole mouse embryo FoxP1 -/-
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 75 kDa
Observed band size: 65,82 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible cross reactivity), 50 kDa (possible cross reactivity), 85 kDa (possible cross reactivity) -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-FOXP1 antibody (ab16645)This image is courtesy of Shunyou Wang, Ph.D.ab16645 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) brain. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/200 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (66)
ab16645 has been referenced in 66 publications.
- Suter TACS et al. TAG-1 Multifunctionality Coordinates Neuronal Migration, Axon Guidance, and Fasciculation. Cell Rep 30:1164-1177.e7 (2020). PubMed: 31995756
- Sheng H et al. Knockdown of FOXP1 promotes the development of lung adenocarcinoma. Cancer Biol Ther 20:537-545 (2019). PubMed: 30409062
- Chaimowicz C et al. Teashirt 1 (Tshz1) is essential for the development, survival and function of hypoglossal and phrenic motor neurons in mouse. Development 146:N/A (2019). PubMed: 31427287
- Iannielli A et al. Reconstitution of the Human Nigro-striatal Pathway on-a-Chip Reveals OPA1-Dependent Mitochondrial Defects and Loss of Dopaminergic Synapses. Cell Rep 29:4646-4656.e4 (2019). PubMed: 31875567
- Nam H et al. Critical roles of ARHGAP36 as a signal transduction mediator of Shh pathway in lateral motor columnar specification. Elife 8:N/A (2019). PubMed: 31305241
Images
-
Western blot - Anti-FOXP1 antibody (ab16645)All lanes : Anti-FOXP1 antibody (ab16645) at 1/5000 dilution
Lane 1 : Lysate from e12.5 whole mouse embryo FoxP1 +/-
Lane 2 : Lysate from e12.5 whole mouse embryo FoxP1 -/-
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 75 kDa
Observed band size: 65,82 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible cross reactivity), 50 kDa (possible cross reactivity), 85 kDa (possible cross reactivity)
-
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-FOXP1 antibody (ab16645) This image is courtesy of Shunyou Wang, Ph.D.ab16645 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) brain. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/200 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG.
