Anti-KAP1 antibody (ab3831)
Key features and details
- Goat polyclonal to KAP1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KAP1 antibody
See all KAP1 primary antibodies -
Description
Goat polyclonal to KAP1 -
Host species
Goat -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide: SSQELSGGPGDGP, corresponding to amino acids 741-753 of KAP 1.
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Positive control
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KAP1 antibody (ab3831)ab3831 staining (0.5
µ g/ml) of HepG2 lysate (RIPA buffer, 30µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab3831 staining (0.5µg/ml) of HepG2 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. -
Immunocytochemistry/ Immunofluorescence - Anti-KAP1 antibody (ab3831)TIF1b is uniformly distributed in the two pronuclei of the mouse embryo at the late zygote stage. Mouse zygotes were processed for immunostaining using the KAP1 antibody. DNA is shown in blue. The dilution used was 1 to 100 in PBS-Tween with 3% BSA. This is part of the review submitted by ME Torres-Padilla on 27 July 2004.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab3831)ab3831 at 2.5 µg/ml staining KAP1 in Human breast tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab3831)ab3831 (4µg/ml) staining of paraffin embedded Human Breast shows nuclear staining in epithelial duct cells. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
