Formalin-fixed, paraffin-embedded human breast carcinoma tissue and serial sections of etoposide treated HeLa cells stained for KAPI (phospho S824) using ab243870 at 1/100 dilution in immunohistochemical analysis. Mock phosphatase treated sections (left) and calf intestinal phosphatase-treated sections (right) are also shown. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243870).

Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with etoposide (right) or untreated (left) labeling KAP-1 (phospho S824) (red) using ab243862 at 1/100 dilution in ICC/IF analysis. DyLight® 594-conjugated goat anti-rabbit IgG was used as the secondary antibody. Counterstain: Phalloidin conjugated Alexa Fluor® 488 (green).

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243870).

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for KAPI (phospho S824) using ab243870 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243870).

KAP1 (phospho S824) was immunoprecipitated from 1 mg HEK-293T whole cell lysate treated with 100 µM etoposide or untreated, using ab243870 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243870 at 1/1000 dilution.

Lane 1: ab243870 IP in HEK-293T cell lysate treated with 100 µM etoposide.

Lane 2: ab243870 Ip in untreated HEK-293T whole cell lysate.

Lane 3: Control IgG in HEK-293T whole cell lysate treated with 100 µM etoposide.

Detection: Chemiluminescence with an exposure time of 10 seconds

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243870).