Anti-5-formylcytosine (5-fC) antibody (ab231898)
Key features and details
- Rabbit polyclonal to 5-formylcytosine (5-fC)
- Suitable for: IP
- Isotype: IgG
Overview
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Product name
Anti-5-formylcytosine (5-fC) antibody -
Description
Rabbit polyclonal to 5-formylcytosine (5-fC) -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Immunogen
Chemical/ Small Molecule corresponding to 5-formylcytosine (5-fC) conjugated to keyhole limpet haemocyanin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-5-formylcytosine (5-fC) antibody (ab231898)
HEK-293 (Human epithelial cell line from embryonic kidney) cells were transfected with a reporter gene and hydroxymethylated in vitro with either a pCAG expression vector containing the TET2 catalytic domain (TET2cd) or a negative control pCAG vector.
DIP assays were performed on 4 µg of sheared and denatured DNA using 3 µl of ab231898 in a total of 500 µl IP buffer. QPCR was performed with primers specifc for the reporter gene. Image shows the recovery, expressed as a % of input (mean +standard deviation of 3 different experiments).