Anti-SLC26A5 antibody [EPR22715-53] - BSA and Azide free (ab269870)
![Anti-SLC26A5 antibody [EPR22715-53] - BSA and Azide free (ab269870)](https://www.abcam.com/ps/products/269/ab269870/Images/ab269870-1-anti-slc26a5-antibody-epr2271553-immunohistochemistry-mouse-cochlear.jpg "Anti-SLC26A5 antibody [EPR22715-53] - BSA and Azide free (ab269870)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22715-53] to SLC26A5 - BSA and Azide free
- Suitable for: IHC - Wholemount
- Reacts with: Mouse
Overview
-
Product name
Anti-SLC26A5 antibody [EPR22715-53] - BSA and Azide free
See all SLC26A5 primary antibodies -
Description
Rabbit monoclonal [EPR22715-53] to SLC26A5 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC - Wmt Mouse
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-Wholemount: Mouse inner ear cochlear tissue.
-
General notes
ab269870 is the carrier-free version of ab242128. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab269870 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22715-53 -
Isotype
IgG -
Research areas
Images
-
IHC - Wholemount - Anti-SLC26A5 antibody [EPR22715-53] - BSA and Azide free (ab269870)
This data was developed using ab242128, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of mouse cochlear tissue labeling SLC26A5 with ab242128 at 1/50000 (0.011µg/ml) dilution.
The image is kindly provided by Dr. Zhiyong Liu, Institute of Neuroscience (ION), CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences (CAS), Shanghai, China.
Prestin, which is encoded by Slc26a5, is specifically expressed in inner ear cochlear outer hair cells (OHCs). Whole-mount prepared cochlear samples were blocked with solution containing 1% Triton X-100, and 5% BSA in 1x PBS for 1 h at room temperature, followed by primary antibody (1:50000) resolved in antibody solution containing 0.1% Triton X-100, and 5% BSA in 1x PBS at 4°C overnight. After 3 times 1x PBS wash, samples were stained with goat anti rabbit secondary antibody conjugated to Alex-Fluor® fluorophores 488 at 1/500 dilution for 4 hrs at room temperature. After 3 times 1x PBS wash, samples were counter-stained with Hoechst 33342 to visualize nuclei. Samples were scanned with Nikon C2 confocal microscope. Counterstained with Hoechst.
-
![Anti-SLC26A5 antibody [EPR22715-53] - BSA and Azide free (ab269870)](https://www.abcam.com/ps/products/269/ab269870/Images/ab269870-2-benefits-of-recombinant-antibodies.png)
Anti-SLC26A5 antibody [EPR22715-53] - BSA and Azide free (ab269870)
