Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PAX8 antibody [SP348] - N-terminal (ab227707)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP348] to PAX8 - N-terminal
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-PAX8 antibody [SP348] - N-terminal
    See all PAX8 primary antibodies

  • Description

    Rabbit monoclonal [SP348] to PAX8 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human PAX8 (N terminal).
    Database link: Q06710

  • Positive control

    • WB: PAX8 transfected HEK-293 cell lysate. IHC-P: Human kidney, uterus, thyroid, fallopian tube, endometrial adenocarcinoma, ovarian adenocarcinoma and renal cell carcinoma tissues. ICC/IF: SK-OV-3Flow Cyt: SK-OV-3, HL-60 cells.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
    Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
    Immunocytochemistry/ Immunofluorescence analysis of SK-OV-3 (human ovarian cancer epithelial cell) cells labeling PAX8 with purified ab227707 at 1:50 (8.1 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
    Western blot - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
    All lanes : Anti-PAX8 antibody [SP348] - N-terminal (ab227707) at 0.398 µg/ml

    Lane 1 : SK-OV-3 (human ovarian cancer epithelial cell) cell lysate
    Lane 2 : human kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

    Predicted band size: 48 kDa

  • Flow Cytometry - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
    Flow Cytometry - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
    Flow Cytometry analysis of SK-OV-3 (human ovarian cancer epithelial cell) cells labeling PAX8 with purified ab227707 at 1:400 dilution (1.015 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)

    Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
    Western blot - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
    All lanes : Anti-PAX8 antibody [SP348] - N-terminal (ab227707) at 1/100 dilution

    Lane 1 : PAX1 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
    Lane 2 : PAX2 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
    Lane 3 : PAX3 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
    Lane 4 : PAX4 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
    Lane 5 : PAX5 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
    Lane 6 : PAX6 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
    Lane 7 : PAX7 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
    Lane 8 : PAX8 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
    Lane 9 : PAX9 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
    Lane 10 : Mock transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate

    Predicted band size: 48 kDa

  • Flow Cytometry - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
    Flow Cytometry - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)

    Flow Cytometry analysis of HL-60 (human promyelocytic leukemia cell line) cells, labeling PAX8 with ab227707 at 1/100 dilution (green) compared to a Rabbit IgG negative control (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)

    Formalin-fixed, paraffin-embedded human kidney tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)

    Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)

    Formalin-fixed, paraffin-embedded human uterus tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)

    Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)

    Formalin-fixed, paraffin-embedded human thyroid tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)

    Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

  • Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
    Anti-PAX8 antibody [SP348] - N-terminal (ab227707)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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