Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PAX8 antibody [PAX8R1] (ab53490)

Key features and details

  • Mouse monoclonal [PAX8R1] to PAX8
  • Suitable for: Flow Cyt, IHC-P, WB, IP, ICC
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-PAX8 antibody [PAX8R1]
    See all PAX8 primary antibodies

  • Description

    Mouse monoclonal [PAX8R1] to PAX8

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant C-terminal fragment corresponding to Human PAX8

  • Positive control

    • HeLa, NIH3T3 or F2408 nuclear cell lysate HeLa cells RIPA lysate of HeLa cells

  • General notes

    IHC-P is not batch-tested and therefore variability between batches might be seen for these applications.

    This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Images

  • Flow Cytometry - Anti-PAX8 antibody [PAX8R1] (ab53490)
    Flow Cytometry - Anti-PAX8 antibody [PAX8R1] (ab53490)

    HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with ab53490 at 0.5 µg/sample (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488-conjugated goat anti-mouse IgG (0.25µg)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [PAX8R1] (ab53490)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [PAX8R1] (ab53490)
    IHC image of ab53490 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53490, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-PAX8 antibody [PAX8R1] (ab53490)
    Western blot - Anti-PAX8 antibody [PAX8R1] (ab53490)
    Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size: 48 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-PAX8 antibody [PAX8R1] (ab53490)
    Western blot - Anti-PAX8 antibody [PAX8R1] (ab53490)
    Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size: 48 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-PAX8 antibody [PAX8R1] (ab53490)
    Western blot - Anti-PAX8 antibody [PAX8R1] (ab53490)
    Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + F2408 whole cell lysate at 25 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size: 48 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry - Anti-PAX8 antibody [PAX8R1] (ab53490)
    Immunocytochemistry - Anti-PAX8 antibody [PAX8R1] (ab53490)
    ab53490, at a 1/10 dilution, staining PAX8 in HeLa cells, using Immunocytochemistry, plus AlexaFluor®488 Goat Anti-mouse IgG secondary anitbody.
  • Immunoprecipitation - Anti-PAX8 antibody [PAX8R1] (ab53490)
    Immunoprecipitation - Anti-PAX8 antibody [PAX8R1] (ab53490)
    Immunoprecipitation: ab53490: 5µg, RIPA lysate of HeLa cells: 500µg. WB: ab53490 at a 1:100 dilution, Secondary antibody - anti-mouse IgG at a 1:1000 dilution

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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