Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µg 314 937 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Mouse monoclonal [PAX8R1] to PAX8
Suitable for: Flow Cyt, IHC-P, WB, IP, ICC
Reacts with: Mouse, Rat, Human
Isotype: IgG1

Product name

Anti-PAX8 antibody [PAX8R1]
See all PAX8 primary antibodies
Description

Mouse monoclonal [PAX8R1] to PAX8
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Human
IHC-P	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant C-terminal fragment corresponding to Human PAX8
Positive control
- HeLa, NIH3T3 or F2408 nuclear cell lysate HeLa cells RIPA lysate of HeLa cells
General notes

IHC-P is not batch-tested and therefore variability between batches might be seen for these applications.

This product was changed from ascites to tissue culture supernatant on 9^th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from TCS
Clonality

Monoclonal
Clone number

PAX8R1
Isotype

IgG1
Research areas

Flow Cytometry - Anti-PAX8 antibody [PAX8R1] (ab53490)

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with ab53490 at 0.5 µg/sample (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488-conjugated goat anti-mouse IgG (0.25µg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [PAX8R1] (ab53490)

IHC image of ab53490 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53490, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-PAX8 antibody [PAX8R1] (ab53490)

Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + HeLa whole cell lysate at 50 µg

Secondary
Mouse IgG antibody at 1/2500 dilution

Predicted band size: 48 kDa
Observed band size: 60 kDa
why is the actual band size different from the predicted?
Western blot - Anti-PAX8 antibody [PAX8R1] (ab53490)

Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

Secondary
Mouse IgG antibody at 1/2500 dilution

Predicted band size: 48 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Western blot - Anti-PAX8 antibody [PAX8R1] (ab53490)

Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + F2408 whole cell lysate at 25 µg

Secondary
Mouse IgG antibody at 1/2500 dilution

Predicted band size: 48 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Immunocytochemistry - Anti-PAX8 antibody [PAX8R1] (ab53490)

ab53490, at a 1/10 dilution, staining PAX8 in HeLa cells, using Immunocytochemistry, plus AlexaFluor®488 Goat Anti-mouse IgG secondary anitbody.
Immunoprecipitation - Anti-PAX8 antibody [PAX8R1] (ab53490)

Immunoprecipitation: ab53490: 5µg, RIPA lysate of HeLa cells: 500µg. WB: ab53490 at a 1:100 dilution, Secondary antibody - anti-mouse IgG at a 1:1000 dilution

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