Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)
![Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)](https://www.abcam.com/ps/products/222/ab222407/Images/ab222407-1-anti-cleaved-n-terminal-dfna5-gsdme-antibody-epr20866-160-western-blot-ct26-mouse.jpg "Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20866-160] to cleaved N-terminal DFNA5 / GSDME
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160]
See all cleaved N-terminal DFNA5 / GSDME primary antibodies -
Description
Rabbit monoclonal [EPR20866-160] to cleaved N-terminal DFNA5 / GSDME -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: CT26 (treated with 2 µg/ml Actinomycin-D for 24 hours) whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20866-160 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)All lanes : Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407) at 1/5000 dilution
Lane 1 : Untreated CT26 (mouse colon carcinoma) whole cell lysate
Lane 2 : CT26 (treated with 2 µg/ml Actinomycin-D for 24 hours) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 57 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 103 secondsLysates were kindly provided by Dr Feng Shao.
The molecular weight observed is consistent with the literature (PMID: 28459430, 30804337).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)](https://www.abcam.com/ps/products/222/ab222407/Images/ab222407-2-benefits-of-recombinant-antibodies.png)
Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407)