Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)
![Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)](https://www.abcam.com/ps/products/251/ab251295/Images/ab251295-408168-anti-tmc2-antibody-epr19446-bsa-and-azide-free-western-blot.jpg "Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19446] to TMC2 - BSA and Azide free
- Suitable for: IHC-Fr, ICC, WB, Flow Cyt
- Reacts with: Mouse
Overview
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Product name
Anti-TMC2 antibody [EPR19446] - BSA and Azide free
See all TMC2 primary antibodies -
Description
Rabbit monoclonal [EPR19446] to TMC2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, ICC, WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251295 is the carrier-free version of ab200039. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251295 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR19446 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)All lanes : Anti-TMC2 antibody [EPR19446] (ab200039) at 1/5000 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with a control vector containing DDDDK tag, whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with DDDDK tagged mouse TMC2 expression vector, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 101 kDa
Observed band size: 101 kDa
Exposure time: 5 secondsThis data was developed using ab200039, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The Cell lysates were kindly provided by our collaborator Dr. Yun Shi. Nanjing University MARC.
The lysates were not boiled prior to loading on the gel.
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![Immunohistochemistry (Frozen sections) - Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)](https://www.abcam.com/ps/products/251/ab251295/Images/ab251295-3-anti-tmc2-antibody-epr19446-bsa-and-azide-free-immunohistochemistry-frozen-sections.jpg)
Immunohistochemistry (Frozen sections) - Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)This data was developed using ab200039, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% PFA-fixed whole mount P21 mouse cochlea tissue labeling TMC2 with ab200039 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (DyLight 488) secondary antibody at 1/500 dilution (green). The results showed cytoplasmic staining on mouse cochlea inner hair cells. Red color: F-actin. The data was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC. -
![Immunocytochemistry - Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)](https://www.abcam.com/ps/products/251/ab251295/Images/ab251295-2-anti-tmc2-antibody-epr19446-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)This data was developed using ab200039, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with DDDDK tagged mouse TMC2 expression vector, labeling TMC2 with ab200039 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HEK-293T cells transfected with DDDDK tagged mouse TMC2 expression vector. The nuclear counter stain is DAPI (blue). FLAG is detected with Anti-FLAG® M2 (red) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) secondary antibody at 1/1000 dilution. The negative controls are as follows: -ve control 1: ab200039 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) secondary antibody at 1/1000 dilution. -ve control 2: Anti-FLAG® M2 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. -
![Flow Cytometry - Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)](https://www.abcam.com/ps/products/251/ab251295/Images/ab251295-1-anti-tmc2-antibody-epr19446-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)This data was developed using ab200039, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with DDDDK tagged mouse TMC2 expression vector cell line labeling TMC2 with ab200039 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. -
![Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)](https://www.abcam.com/ps/products/251/ab251295/Images/ab251295-5-benefits-of-recombinant-antibodies.png)
Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)