Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19446] to TMC2 - BSA and Azide free
  • Suitable for: IHC-Fr, ICC, WB, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-TMC2 antibody [EPR19446] - BSA and Azide free
    See all TMC2 primary antibodies

  • Description

    Rabbit monoclonal [EPR19446] to TMC2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, ICC, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251295 is the carrier-free version of ab200039. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251295 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)
    Western blot - Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)
    All lanes : Anti-TMC2 antibody [EPR19446] (ab200039) at 1/5000 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with a control vector containing DDDDK tag, whole cell lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) transfected with DDDDK tagged mouse TMC2 expression vector, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 101 kDa
    Observed band size: 101 kDa


    Exposure time: 5 seconds


    This data was developed using ab200039, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The Cell lysates were kindly provided by our collaborator Dr. Yun Shi. Nanjing University MARC.

    The lysates were not boiled prior to loading on the gel.

  • Immunohistochemistry (Frozen sections) - Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)
    Immunohistochemistry (Frozen sections) - Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)
    This data was developed using ab200039, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% PFA-fixed whole mount P21 mouse cochlea tissue labeling TMC2 with ab200039 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (DyLight 488) secondary antibody at 1/500 dilution (green). The results showed cytoplasmic staining on mouse cochlea inner hair cells. Red color: F-actin. The data was kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.
  • Immunocytochemistry - Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)
    Immunocytochemistry - Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)
    This data was developed using ab200039, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with DDDDK tagged mouse TMC2 expression vector, labeling TMC2 with ab200039 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HEK-293T cells transfected with DDDDK tagged mouse TMC2 expression vector. The nuclear counter stain is DAPI (blue). FLAG is detected with Anti-FLAG® M2 (red) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) secondary antibody at 1/1000 dilution. The negative controls are as follows: -ve control 1: ab200039 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) secondary antibody at 1/1000 dilution. -ve control 2: Anti-FLAG® M2 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
  • Flow Cytometry - Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)
    Flow Cytometry - Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)
    This data was developed using ab200039, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with DDDDK tagged mouse TMC2 expression vector cell line labeling TMC2 with ab200039 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
  • Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)
    Anti-TMC2 antibody [EPR19446] - BSA and Azide free (ab251295)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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