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Anti-ITM2B antibody [OTI1C11] (ab119044)

Key features and details

  • Mouse monoclonal [OTI1C11] to ITM2B
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-ITM2B antibody [OTI1C11]
    See all ITM2B primary antibodies

  • Description

    Mouse monoclonal [OTI1C11] to ITM2B

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length Human ITM2B produced in HEK293T cells (NP_068839).

  • Positive control

    • HEK293T cell lysate transfected with pCMV6-ENTRY ITM2B cDNA; Human kidney tissue; HeLa and Jurkat cells.

  • General notes

    Dilute in PBS (pH7.3) before use. 

    The clone number has been updated from 1C11 to OTI1C11, both clone numbers name the same clone.

     

    This product was changed from ascites to tissue culture supernatant on 5th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Western blot - Anti-ITM2B antibody [OTI1C11] (ab119044)
    Western blot - Anti-ITM2B antibody [OTI1C11] (ab119044)
    All lanes : Anti-ITM2B antibody [OTI1C11] (ab119044) at 1/2000 dilution

    Lane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY ITM2B cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 30 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202377)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITM2B antibody [OTI1C11] (ab119044)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITM2B antibody [OTI1C11] (ab119044)
    ab119044, at 1/150 dilution, staining ITM2B in paraffin embedded Human kidney by Immunohistochemistry.
  • Flow Cytometry - Anti-ITM2B antibody [OTI1C11] (ab119044)
    Flow Cytometry - Anti-ITM2B antibody [OTI1C11] (ab119044)
    ab119044 at 1/100 dilution staining ITM2B in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
  • Flow Cytometry - Anti-ITM2B antibody [OTI1C11] (ab119044)
    Flow Cytometry - Anti-ITM2B antibody [OTI1C11] (ab119044)
    ab119044 at 1/100 dilution staining ITM2B in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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