Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-PAX8 antibody [EPR23508-20] - BSA and Azide free
See all PAX8 primary antibodies
Description

Rabbit monoclonal [EPR23508-20] to PAX8 - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Human
IHC-Fr	Mouse
IHC-P	Mouse
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
ab275259 is the carrier-free version of ab239363. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab275259 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C.
Storage buffer

Constituent: 100% PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23508-20
Isotype

IgG
Research areas

Western blot - Anti-PAX8 antibody [EPR23508-20] - BSA and Azide free (ab275259)

Anti-PAX8 antibody [EPR23508-20] (ab239363) at 1/1000 dilution + Mouse E18 kidney tissue lysate at 60 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 48 kDa

ab239363, the same antibody clone in a different buffer formulation.
> Blocking and diluting buffer and concentration: The molecular weight observed is consistent with what has been described in the literature (PMID: 21602887)
This blot was developed using a higher sensitivity ECL substrate.

3 minutes

Exposure time:
Western blot - Anti-PAX8 antibody [EPR23508-20] - BSA and Azide free (ab275259)

All lanes : Anti-PAX8 antibody [EPR23508-20] (ab239363) at 1/1000 dilution

Lane 1 : SK-OV-3 (human ovarian cancer epithelial cell), whole cell lysate
Lane 2 : NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 48 kDa

ab239363, the same antibody clone in a different buffer formulation.
> Blocking and diluting buffer and concentration: The antibody detects isoform of PAX8
The molecular weight observed is consistent with what has been described in the literature (PMID: 21602887)

This blot was developed using a higher sensitivity ECL substrate.

Fresh lysate was used in this Lane 2.

Lane 1: 127 seconds Lane 2: 59 seconds

Exposure time:
Immunohistochemistry (Frozen sections) - Anti-PAX8 antibody [EPR23508-20] - BSA and Azide free (ab275259)

This data was developed using ab239363, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling PAX8 with ab239363 at 1/50 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Nuclear staining on mouse kidney. is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunoprecipitation - Anti-PAX8 antibody [EPR23508-20] - BSA and Azide free (ab275259)

This data was developed using ab239363, the same antibody clone in a different buffer formulation.
PAX8 was immunoprecipitated from 0.35 mg SK-OV-3 (human ovarian cancer epithelial cell), whole cell lysate 10 ug with ab239363 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239363 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: SK-OV-3 (human ovarian cancer epithelial cell), whole cell lysate 10 ug

Lane 2: abab239363 IP in SK-OV-3 whole cell lysate

Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab239363 in SK-OV-3 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 49 seconds

This blot was developed using a higher sensitivity ECL substrate.
Flow Cytometry - Anti-PAX8 antibody [EPR23508-20] - BSA and Azide free (ab275259)

This data was developed using ab239363, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell) cells labelling PAX8 with ab239363 at 1/500 dilution (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunocytochemistry - Anti-PAX8 antibody [EPR23508-20] - BSA and Azide free (ab275259)

This data was developed using ab239363, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-OV-3 (human ovarian cancer epithelial cell) cells labelling PAX8 with ab239363 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in SK-OV-3 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Immunocytochemistry - Anti-PAX8 antibody [EPR23508-20] - BSA and Azide free (ab275259)

This data was developed using ab239363, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell) cells labelling PAX8 with ab239363 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (Green). Confocal image showing nuclear staining in NIH:OVCAR-3 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR23508-20] - BSA and Azide free (ab275259)

This data was developed using ab239363, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling PAX8 with abab239363 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in human ovarian cancer (PMID: 21317881). The section was incubated with ab239363 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR23508-20] - BSA and Azide free (ab275259)

This data was developed using ab239363, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human thyroid tissue labeling PAX8 with abab239363 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in human thyroid (PMID: 21317881). The section was incubated with ab239363 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR23508-20] - BSA and Azide free (ab275259)

This data was developed using ab239363, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue labeling PAX8 with abab239363 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in mouse thyroid (PMID: 21317881). The section was incubated with ab239363 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Anti-PAX8 antibody [EPR23508-20] - BSA and Azide free (ab275259)