Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)

Key features and details

  • Rabbit monoclonal [SP348] to PAX8 - BSA and Azide free
  • Suitable for: ICC/IF, WB, Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-PAX8 antibody [SP348] - BSA and Azide free
    See all PAX8 primary antibodies

  • Description

    Rabbit monoclonal [SP348] to PAX8 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog

  • Immunogen

    Synthetic peptide within Human PAX8 (N terminal). The exact sequence is proprietary.
    Database link: Q06710

  • Positive control

    • WB: PAX8 transfected HEK-293 cell lysate. IHC-P: Human kidney, uterus, thyroid, fallopian tube, endometrial adenocarcinoma, ovarian adenocarcinoma and renal cell carcinoma tissues. ICC/IF: SK-OV-3Flow Cyt: SK-OV-3, HL-60 cells.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab242429 is the carrier-free version of ab227707. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab242429 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
    Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
    Immunocytochemistry/ Immunofluorescence analysis of SK-OV-3 (human ovarian cancer epithelial cell) cells labeling PAX8 with purified ab227707 at 1:50 (8.1 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242429)
  • Flow Cytometry - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
    Flow Cytometry - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
    Flow Cytometry analysis of SK-OV-3 (human ovarian cancer epithelial cell) cells labeling PAX8 with purified ab227707 at 1:400 dilution (1.015 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242429)
  • Flow Cytometry - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
    Flow Cytometry - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)

    Flow Cytometry analysis of HL-60 (human promyelocytic leukemia cell line) cells, labeling PAX8 with ab227707 at 1/100 dilution (green) compared to a Rabbit IgG negative control (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227707).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)

    Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227707).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)

    Formalin-fixed, paraffin-embedded human kidney tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227707).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)

    Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227707).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)

    Formalin-fixed, paraffin-embedded human uterus tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227707).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)

    Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227707).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)

    Formalin-fixed, paraffin-embedded human thyroid tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227707).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)

    Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227707).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227707).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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