Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] - BSA and Azide free (ab256165)
![Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] - BSA and Azide free (ab256165)](https://www.abcam.com/ps/products/256/ab256165/Images/ab256165-1-anti-cleaved-n-terminal-dfna5-gsdme-antibody-epr20867-248-western-blot-sh-sy5y-human.jpg "Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] - BSA and Azide free (ab256165)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20867-248] to cleaved N-terminal DFNA5 / GSDME - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] - BSA and Azide free
See all cleaved N-terminal DFNA5 / GSDME primary antibodies -
Description
Rabbit monoclonal [EPR20867-248] to cleaved N-terminal DFNA5 / GSDME - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SH-SY5Y (treated with etoposide (60uM, 14h) whole cell lysate.
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General notes
Ab256165 is the carrier-free version of ab222408. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256165 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20867-248 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DFNA5/GSDME antibody [EPR20867-248] - BSA and Azide free (ab256165)All lanes : Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] (ab222408) at 1/1000 dilution
Lane 1 : Sample: Wild-type SH-SY5Y (human neuroblastoma cell line from bone marrow) (treated with treated with etoposide (60uM, 14h), whole cell lysate, 20 ug
Lane 2 : Untreated wild-type SH-SY5Y whole cell lysate, 20 ug
Lane 3 : DFNA5/GSDME knockout SH-SY5Y treated with etoposide (60 uM, 14h), whole cell lysate, 20 ug
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 55 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Lysates were kindly provided by Dr Feng Shao.
The molecular weight observed is consistent with the literature (PMID: 28459430, 30804337).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222408).
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![Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] - BSA and Azide free (ab256165)](https://www.abcam.com/ps/products/256/ab256165/Images/ab256165-2-benefits-of-recombinant-antibodies.png)
Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] - BSA and Azide free (ab256165)
