Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DFNA5/GSDME antibody [EPR19859] - BSA and Azide free (ab223877)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19859] to DFNA5/GSDME - BSA and Azide free
  • Suitable for: IP, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DFNA5/GSDME antibody [EPR19859] - BSA and Azide free
    See all DFNA5/GSDME primary antibodies

  • Description

    Rabbit monoclonal [EPR19859] to DFNA5/GSDME - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Expression levels of the target protein vary with sample type and some optimisation may be required.

  • Tested applications

    Suitable for: IP, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SH-SY5Y and EMT6 whole cell lysates; Human fetal brain lysate; Mouse and rat brain lysates; HEK-293 transfected with DDDDK tagged DFNA5/GSDME (N-terminal) expression vector whole cell lysate. Flow Cyt.: SH-SY5Y cells.

  • General notes

    Ab223877 is the carrier-free version of ab215191. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab223877 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19859

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - BSA and Azide free (ab223877)
    Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - BSA and Azide free (ab223877) Image from Wang Y et al., Biochem Biophys Res Commu. 2018;495(1):1418-1425. Fig 4(A).; 10.1016/j.bbrc.2017.11.156 with permission from Elsevier.
    All lanes : Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (ab215191) at 1/1000 dilution

    Lane 1 : Wild Type SGC-7901 cell lysates
    Lane 2 : DFNA5/GSDME Knockout SGC-7901 cell lysates

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

    Predicted band size: 55 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215191).

    https://www.sciencedirect.com/journal/biochemical-and-biophysical-research-communications

  • Immunoprecipitation - Anti-DFNA5/GSDME antibody [EPR19859] - BSA and Azide free (ab223877)
    Immunoprecipitation - Anti-DFNA5/GSDME antibody [EPR19859] - BSA and Azide free (ab223877)

    ab215191 at 1/30 immunoprecipitating DFNA5/GSDME in EMT6 whole cell lysate.

    Lane 1 (input): EMT6 whole cell lysate (10μg)

    Lane 2 (+): ab215191 + EMT6 whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of  ab215191 in EMT6 whole cell lysate

    For western blotting, ab215191 at 1/500 and ab131366 VeriBlot for IP Detection Reagent (HRP) was used at 1/1000.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    EMT6 cells were kindly provided by our collaborator Dr. Feng Shao, NIBS

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215191).

  • Flow Cytometry - Anti-DFNA5/GSDME antibody [EPR19859] - BSA and Azide free (ab223877)
    Flow Cytometry - Anti-DFNA5/GSDME antibody [EPR19859] - BSA and Azide free (ab223877)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized EMT6 cell line labeling DFNA5/GSDME with ab215191 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    EMT6 cells were kindly provided by our collaborator Dr. Feng Shao, NIBS

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215191).

  • Flow Cytometry - Anti-DFNA5/GSDME antibody [EPR19859] - BSA and Azide free (ab223877)
    Flow Cytometry - Anti-DFNA5/GSDME antibody [EPR19859] - BSA and Azide free (ab223877)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cell line labeling DFNA5/GSDME  with ab215191 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215191).

  • Flow Cytometry - Anti-DFNA5/GSDME antibody [EPR19859] - BSA and Azide free (ab223877)
    Flow Cytometry - Anti-DFNA5/GSDME antibody [EPR19859] - BSA and Azide free (ab223877)

    This Flow Cyt data was generated using the same anti-DFNA5/GSDME antibody clone [EPR19859] in a different buffer formulation (cat# ab215191).

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cell line labeling DFNA5/GSDME  with ab215191 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Anti-DFNA5/GSDME antibody [EPR19859] - BSA and Azide free (ab223877)
    Anti-DFNA5/GSDME antibody [EPR19859] - BSA and Azide free (ab223877)

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