Anti-DFNA5/GSDME antibody [EPR20866-160] - BSA and Azide free (ab256122)
![Anti-DFNA5/GSDME antibody [EPR20866-160] - BSA and Azide free (ab256122)](https://www.abcam.com/ps/products/256/ab256122/Images/ab256122-349784-anticleavednterminaldfna5gsdmeantibodyepr20866160westernblotct26mouse.jpg "Anti-DFNA5/GSDME antibody [EPR20866-160] - BSA and Azide free (ab256122)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20866-160] to DFNA5/GSDME - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-DFNA5/GSDME antibody [EPR20866-160] - BSA and Azide free
See all DFNA5/GSDME primary antibodies -
Description
Rabbit monoclonal [EPR20866-160] to DFNA5/GSDME - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: CT26 (treated with 2 µg/ml Actinomycin-D for 24 hours) whole cell lysate.
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General notes
Ab256122 is the carrier-free version of ab222407. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256122 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20866-160 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DFNA5/GSDME antibody [EPR20866-160] - BSA and Azide free (ab256122)All lanes : Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20866-160] (ab222407) at 1/1000 dilution
Lane 1 : Untreated CT26 (mouse colon carcinoma) whole cell lysate
Lane 2 : CT26 (treated with 2 µg/ml Actinomycin-D for 24 hours) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 100000 cells
Predicted band size: 54 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 103 secondsLysates were kindly provided by Dr Feng Shao.
The molecular weight observed is consistent with the literature (PMID: 28459430, 30804337).
Blocking/Dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222407).
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![Anti-DFNA5/GSDME antibody [EPR20866-160] - BSA and Azide free (ab256122)](https://www.abcam.com/ps/products/256/ab256122/Images/ab256122-2-benefits-of-recombinant-antibodies.png)
Anti-DFNA5/GSDME antibody [EPR20866-160] - BSA and Azide free (ab256122)
