Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5335] to Myosin, smooth muscle heavy chain 1 and 2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335]
    See all Myosin, smooth muscle heavy chain 1 and 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR5335] to Myosin, smooth muscle heavy chain 1 and 2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Myosin, smooth muscle heavy chain 1 and 2. The exact sequence is proprietary.

  • Positive control

    • WB: Human fetal artery, bladder and mouse spleen lysates IHC: Human colon and kidney tissues

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 1.85 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5335

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)
    Western blot - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)
    Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679) at 1/20000 dilution (purified) + mouse spleen at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 227 kDa
    Observed band size: 250 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)
    Western blot - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)
    Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679) at 1/50000 dilution (purified) + human fetal artery lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 227 kDa
    Observed band size: 250 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)
    Immunohistochemical staining of paraffin embedded human smooth muscle with purified ab124679 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)
    Immunohistochemical staining of paraffin embedded human skeletal muscle with purified ab124679 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)

    Unpurified ab124679, at 1/100 dilution, staining Myosin, smooth muscle heavy chain 1 and 2 in paraffin-embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)

    Unpurified ab124679, at 1/100 dilution, staining Myosin, smooth muscle heavy chain 1 and 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)
    Western blot - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)
    All lanes : Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679) at 1/10000 dilution (unpurified)

    Lane 1 : Human fetal artery lysate
    Lane 2 : Human bladder lysate
    Lane 3 : Mouse spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 227 kDa
    Observed band size: 250 kDa why is the actual band size different from the predicted?

  • OI-RD Scanning - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)
    OI-RD Scanning - Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)
    Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)

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