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Neuroscience Sensory System Auditory system

Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843)

Price and availability

288 134 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19859-60] to DFNA5/GSDME - C-terminal
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal
    See all DFNA5/GSDME primary antibodies
  • Description

    Rabbit monoclonal [EPR19859-60] to DFNA5/GSDME - C-terminal
  • Host species

    Rabbit
  • Specificity

    Expression levels of the target protein vary with sample type and some optimisation may be required.

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate. HEK-293 transfected with a myc tagged, DFNA5/GSDME (C-terminus) expression vector, whole cell lysate. Human fetal brain lysate. IP: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19859-60
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Sensory System
    • Auditory system

Images

  • Western blot - Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843)
    Western blot - Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843)
    All lanes : Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843) at 1/5000 dilution

    Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
    Lane 2 : DFNA5/GSDME knockout SH-SY5Y, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 54 kDa


    Exposure time: 30 seconds


    Blocking/Dilution: 5% NFDM/TBST.

    The cell lysates were kindly provided by our collaborator, Dr. Feng Shao, NIBS.

  • Western blot - Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843)
    Western blot - Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843)
    All lanes : Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843) at 1/1000 dilution

    Lane 1 : HEK-293 (human embryonic kidney epithelial cell) transfected with a control vector containing DDDDK tag, whole cell lysate
    Lane 2 : HEK-293 transfected with a DDDDK tagged, DFNA5/GSDME (N-terminus) expression vector, whole cell lysate
    Lane 3 : HEK-293 transfected with a myc tagged, DFNA5/GSDME (C-terminus) expression vector, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa


    Exposure time: 5 seconds


    Blocking/Dilution: 5% NFDM/TBST .

    This antibody is specific to the C-terminus of DFNA5/GSDME.

    The cell lysates were kindly provided by our collaborator, Dr. Feng Shao, NIBS.

  • Western blot - Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843)
    Western blot - Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843)
    Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843) at 1/1000 dilution + human fetal brain at 10 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa


    Exposure time: 3 minutes


    Blocking/Dilution: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 28459430).

  • Immunoprecipitation - Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843)
    Immunoprecipitation - Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843)

    DFNA5/GSDME was immunoprecipitated from 0.35 mg of SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with ab221843 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab221843 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
    Lane 1: SH-SY5Y whole cell lysate 10ug (Input).
    Lane 2: ab221843 IP in SH-SY5Y whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221843 in SH-SY5Y whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Observed MW 55 kDa.
    Exposure time: 1 second.

  • Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843)
    Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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