Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DFNA5/GSDME antibody [EPR19859-60] - BSA and Azide free (ab225893)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19859-60] to DFNA5/GSDME - BSA and Azide free
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-DFNA5/GSDME antibody [EPR19859-60] - BSA and Azide free
    See all DFNA5/GSDME primary antibodies

  • Description

    Rabbit monoclonal [EPR19859-60] to DFNA5/GSDME - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SH-SY5Y whole cell lysate.

  • General notes

    Ab225893 is the carrier-free version of ab221843. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab225893 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19859-60

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-DFNA5/GSDME antibody [EPR19859-60] - BSA and Azide free (ab225893)
    Immunoprecipitation - Anti-DFNA5/GSDME antibody [EPR19859-60] - BSA and Azide free (ab225893)

    DFNA5/GSDME was immunoprecipitated from 0.35 mg of SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with ab221843 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab221843 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
    Lane 1: SH-SY5Y whole cell lysate 10ug (Input).
    Lane 2: ab221843 IP in SH-SY5Y whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221843 in SH-SY5Y whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Observed MW 55 kDa.
    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221843).

  • Western blot - Anti-DFNA5/GSDME antibody [EPR19859-60] - BSA and Azide free (ab225893)
    Western blot - Anti-DFNA5/GSDME antibody [EPR19859-60] - BSA and Azide free (ab225893)
    All lanes : Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843) at 1/5000 dilution

    Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
    Lane 2 : DFNA5/GSDME knockout SH-SY5Y, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 54 kDa


    Exposure time: 30 seconds


    Blocking/Dilution: 5% NFDM/TBST.

    The cell lysates were kindly provided by our collaborator, Dr. Feng Shao, NIBS.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221843).

  • Anti-DFNA5/GSDME antibody [EPR19859-60] - BSA and Azide free (ab225893)
    Anti-DFNA5/GSDME antibody [EPR19859-60] - BSA and Azide free (ab225893)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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