Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14394] to RBMX2 - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RBMX2 antibody [EPR14394] - BSA and Azide free
    See all RBMX2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14394] to RBMX2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250272 is the carrier-free version of ab180940. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250272 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)
    Immunocytochemistry - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)

    This data was developed using ab180940, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cell line staining RBMX2 using ab180940 at a 1/100 dilution. Goat anti rabbit IgG (Dylight 488), at a dilution of 1/200, was used as a secondary antibody. Counter stain: Dapi (blue).
  • Western blot - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)
    Western blot - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)
    All lanes : Anti-RBMX2 antibody [EPR14394] (ab180940) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa



    This data was developed using ab180940, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)
    Western blot - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)
    Anti-RBMX2 antibody [EPR14394] (ab180940) at 1/2000 dilution + HT-1376 at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa



    This data was developed using ab180940, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)
    This data was developed using ab180940, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling RBMX2 with ab180940 at a 1/100 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)
    This data was developed using ab180940, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling RBMX2 with ab180940 at a 1/100 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)
    Immunocytochemistry - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)
    This data was developed using ab180940, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of -20? acetone-fixed HeLa cell line staining RBMX2 using ab180940 at a 1/100 dilution. Goat anti rabbit IgG (Dylight 488), at a dilution of 1/200, was used as a secondary antibody. Counter stain: Dapi (blue).
  • Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)
    Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)

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