Anti-Topoisomerase II beta/TOP2B antibody (ab264158)
Key features and details
- Rabbit polyclonal to Topoisomerase II beta/TOP2B
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Topoisomerase II beta/TOP2B antibody
See all Topoisomerase II beta/TOP2B primary antibodies -
Description
Rabbit polyclonal to Topoisomerase II beta/TOP2B -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Topoisomerase II beta/TOP2B aa 1570-1621 (C terminal). The exact sequence is proprietary. NP_001059.2
Database link: Q02880 -
Positive control
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human small cell lung cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Topoisomerase II beta/TOP2B antibody (ab264158)All lanes : Anti-Topoisomerase II beta/TOP2B antibody (ab264158) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Predicted band size: 183 kDa
Exposure time: 30 seconds4-8% SDS-PAGE
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Immunoprecipitation - Anti-Topoisomerase II beta/TOP2B antibody (ab264158)Topoisomerase II beta/TOP2B was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264158 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264158 at 0.1 µg/ml.
Lane 1: ab264158 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 10 secs.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II beta/TOP2B antibody (ab264158)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human small cell lung cancer tissue labeling Topoisomerase II beta/TOP2B with ab264158 at 1/200 dilution. Detection: DAB.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
