Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bcl-2 antibody [10C4] - BSA and Azide free (ab252259)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [10C4] to Bcl-2 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Bcl-2 antibody [10C4] - BSA and Azide free
    See all Bcl-2 primary antibodies

  • Description

    Mouse monoclonal [10C4] to Bcl-2 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3 whole cell lysate. Mouse thymus and spleen tissue lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab252259 is the carrier-free version of ab16904. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Images

  • Western blot - Anti-Bcl-2 antibody [10C4] - BSA and Azide free (ab252259)
    Western blot - Anti-Bcl-2 antibody [10C4] - BSA and Azide free (ab252259)
    All lanes : Anti-Bcl-2 antibody [10C4] (ab16904) at 0.488 µg/ml

    Lane 1 : Mouse thymus tissue lysate
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 3 : Mouse spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 26 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 15 secs; Lane 2,3: 180 secs.

    This image was produced using the same antibody clone but in a different formulation ab16904, PBS, sodium azide, glycerol and BSA.

  • Anti-Bcl-2 antibody [10C4] - BSA and Azide free (ab252259)
    Anti-Bcl-2 antibody [10C4] - BSA and Azide free (ab252259)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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