Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CBX4 antibody [EPR23053-7] - ChIP Grade – BSA and Azide free (ab269462)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23053-7] to CBX4 - ChIP Grade – BSA and Azide free
  • Suitable for: ChIP, IHC-P, WB, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CBX4 antibody [EPR23053-7] - ChIP Grade – BSA and Azide free
    See all CBX4 primary antibodies

  • Description

    Rabbit monoclonal [EPR23053-7] to CBX4 - ChIP Grade – BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    Flow Cyt
    Human
    IHC-P
    Rat
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HCT 116, RAW 264.7, C6, HDLM-2, NCCIT, Mouse thymus and Rat thymus lysates. IHC-P: Mouse cerebrum, Mouse hippocampus and Rat hippocampus tissues. Flow Cyt: HCT 116 and NCCIT cells. IP: HCT 116 whole cell lysate. ChIP: Chromatin prepared from NCCIT cells.

  • General notes

    ab269462 is the carrier-free version of ab242149. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab269462 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • ChIP - Anti-CBX4 antibody [EPR23053-7] - BSA and Azide free (ab269462)
    ChIP - Anti-CBX4 antibody [EPR23053-7] - BSA and Azide free (ab269462)

    Chromatin was prepared from NCCIT cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then 1% formaldehyde for 10min.

    The ChIP was performed with 25 µg of chromatin, 2 µg of ab242149 (red), or 2 µg of rabbit normal IgG ab172730 (gray) and 20 µl of Protein A/G sepharose beads.  The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).

    *https://www.abcam.com/resources?keywords=X%20ChIP%20protocol

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242149).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX4 antibody [EPR23053-7] - BSA and Azide free (ab269462)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX4 antibody [EPR23053-7] - BSA and Azide free (ab269462)

    Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling CBX4 with ab242149 at 1/200 dilution (2.785 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on rat hippocampus (PMID: 28283560). The section was incubated with ab242149 for 30 at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242149).

  • Immunoprecipitation - Anti-CBX4 antibody [EPR23053-7] - BSA and Azide free (ab269462)
    Immunoprecipitation - Anti-CBX4 antibody [EPR23053-7] - BSA and Azide free (ab269462)

    CBX4 was immunoprecipitated from 0.35 mg HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate with ab242149 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab242149 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate 10ug

    Lane 2: ab242149 IP in HCT 116 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242149 in HCT 116 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242149).

  • Flow Cytometry - Anti-CBX4 antibody [EPR23053-7] - BSA and Azide free (ab269462)
    Flow Cytometry - Anti-CBX4 antibody [EPR23053-7] - BSA and Azide free (ab269462)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labelling CBX4 with ab242149 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242149).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX4 antibody [EPR23053-7] - BSA and Azide free (ab269462)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX4 antibody [EPR23053-7] - BSA and Azide free (ab269462)

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling CBX4 with ab242149 at 1/200 dilution (2.785 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on mouse hippocampus (PMID: 28283560). The section was incubated with ab242149 for 30 at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242149).

  • Flow Cytometry - Anti-CBX4 antibody [EPR23053-7] - BSA and Azide free (ab269462)
    Flow Cytometry - Anti-CBX4 antibody [EPR23053-7] - BSA and Azide free (ab269462)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HCT 116 (Human colorectal carcinoma epithelial cell) cells labelling CBX4 with ab242149 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242149).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX4 antibody [EPR23053-7] - BSA and Azide free (ab269462)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBX4 antibody [EPR23053-7] - BSA and Azide free (ab269462)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling CBX4 with ab242149 at 1/200 dilution (2.785 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Nuclear staining on mouse cerebrum (PMID: 28283560). The section was incubated with ab242149 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242149).

  • Anti-CBX4 antibody [EPR23053-7] - ChIP Grade – BSA and Azide free (ab269462)
    Anti-CBX4 antibody [EPR23053-7] - ChIP Grade – BSA and Azide free (ab269462)

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