Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] - BSA and Azide free (ab249091)
![Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] - BSA and Azide free (ab249091)](https://www.abcam.com/ps/products/249/ab249091/Images/ab249091-406429-anti-hp1-gamma-cbx3-antibody-epr10466-b-bsa-and-azide-free-western-blot.jpg "Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] - BSA and Azide free (ab249091)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10466(B)] to HP1 gamma/CBX3 - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] - BSA and Azide free
See all HP1 gamma/CBX3 primary antibodies -
Description
Rabbit monoclonal [EPR10466(B)] to HP1 gamma/CBX3 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249091 is the carrier-free version of ab154195 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab249091 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information. Mouse: In our hands this product works best in WB using cell lines rather than tissue lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10466(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] - BSA and Azide free (ab249091)
This data was developed using ab154195, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HP1 gamma/CBX3 knockout HAP1 cell lysate (20 µg)
Lane 3: NIH3T3 cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab154195 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab154195 was shown to recognize HP1 gamma/CBX3 when HP1 gamma/CBX3 knockout samples were used, along with additional cross-reactive bands. Wild-type and HP1 gamma/CBX3 knockout samples were subjected to SDS-PAGE. ab154195 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] - BSA and Azide free (ab249091)](https://www.abcam.com/ps/products/249/ab249091/Images/ab249091-402361-anti-hp1-gamma-cbx3-antibody-epr10466-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] - BSA and Azide free (ab249091)All lanes : Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : MOLT4 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 20 kDaThis data was developed using ab154195, the same antibody clone in a different buffer formulation.
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![Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] - BSA and Azide free (ab249091)](https://www.abcam.com/ps/products/249/ab249091/Images/ab249091-3-benefits-of-recombinant-antibodies.png)
Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] - BSA and Azide free (ab249091)
