Anti-Pdd1 antibody (ab5338)
Key features and details
- Rabbit polyclonal to Pdd1
- Suitable for: WB, ICC
- Reacts with: Tetrahymena
- Isotype: IgG
Overview
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Product name
Anti-Pdd1 antibody -
Description
Rabbit polyclonal to Pdd1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Tetrahymena
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Immunogen
Synthetic peptide corresponding to Tetrahymena Pdd1 aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab24392)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB TetrahymenaApplication Abreviews Notes WB (1) 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 57 kDa).ICC Use at an assay dependent concentration.Notes WB
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 57 kDa).ICC
Use at an assay dependent concentration.Target
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Relevance
The PDD1 gene sequence predicts a novel polypeptide containing two copies of a chromodomain, a conserved domain found in a number of heterochromatin-associated proteins (Koonin et al. 1995). Consistent with this finding, Pdd1p localizes to electron-dense heterochromatic structures that concentrate at the periphery of macronuclear anlagen during the time when DNA deletion occurs. -
Cellular localization
Nuclear -
Database links
- SwissProt: Q94996 Tetrahymena
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Alternative names
- p65 antibody
- Pdd 1 antibody
- Pdd 1p antibody
see all
Images
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Immunocytochemistry - Anti-Pdd1 antibody (ab5338)This image is courtesy of an Anonymous Abreview.ab5338 staining Pdd1 in wild-type Tetrahymena sp. cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in methanol. The primary antibody was diluted 1/250 in TBS + 1% Goat Serum and incubated with the sample for 18 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/1000.
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Western blot - Anti-Pdd1 antibody (ab5338)This image is courtesy of an anonymous Abreview.All lanes : Anti-Pdd1 antibody (ab5338) at 1/1000 dilution
Lane 1 : Wild-type Tetrahymena sp. Starved Tetrahymena, do not express pdd1p (hour 0)
Lane 2 : Wild-type Tetrahymena sp. Conjugating Tetrahymena, peak of pdd1p expression (hour 9)
Lysates/proteins at 15000 cells per lane.
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Datasheets and documents
References (17)
ab5338 has been referenced in 17 publications.
- Garg J et al. The Med31 Conserved Component of the Divergent Mediator Complex in Tetrahymena thermophila Participates in Developmental Regulation. Curr Biol 29:2371-2379.e6 (2019). PubMed: 31280994
- Noto T & Mochizuki K Small RNA-Mediated trans-Nuclear and trans-Element Communications in Tetrahymena DNA Elimination. Curr Biol 28:1938-1949.e5 (2018). PubMed: 29887308
- Kataoka K & Mochizuki K Heterochromatin aggregation during DNA elimination in Tetrahymena is facilitated by a prion-like protein. J Cell Sci 130:480-489 (2017). ICC/IF ; Tetrahymena . PubMed: 27909245
- Farley BM & Collins K Transgenerational function of Tetrahymena Piwi protein Twi8p at distinctive noncoding RNA loci. RNA 23:530-545 (2017). PubMed: 28053272
- Suhren JH et al. Negative Regulators of an RNAi-Heterochromatin Positive Feedback Loop Safeguard Somatic Genome Integrity in Tetrahymena. Cell Rep 18:2494-2507 (2017). PubMed: 28273462
Images
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Immunocytochemistry - Anti-Pdd1 antibody (ab5338) This image is courtesy of an Anonymous Abreview.ab5338 staining Pdd1 in wild-type Tetrahymena sp. cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in methanol. The primary antibody was diluted 1/250 in TBS + 1% Goat Serum and incubated with the sample for 18 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/1000.
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Western blot - Anti-Pdd1 antibody (ab5338) This image is courtesy of an anonymous Abreview.All lanes : Anti-Pdd1 antibody (ab5338) at 1/1000 dilution
Lane 1 : Wild-type Tetrahymena sp. Starved Tetrahymena, do not express pdd1p (hour 0)
Lane 2 : Wild-type Tetrahymena sp. Conjugating Tetrahymena, peak of pdd1p expression (hour 9)
Lysates/proteins at 15000 cells per lane.
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds