Anti-KAP1 antibody [20C1] (ab22553)
![Anti-KAP1 antibody [20C1] (ab22553)](https://www.abcam.com/ps/products/22/ab22553/Images/ab22553-32063-anti-kap1-antibody-20c1-chip-grade-immunohistochemistry.jpg "Anti-KAP1 antibody [20C1] (ab22553)")
Key features and details
- Mouse monoclonal [20C1] to KAP1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-KAP1 antibody [20C1]
See all KAP1 primary antibodies -
Description
Mouse monoclonal [20C1] to KAP1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Recombinant fragment, corresponding to amino acids 60-383 of Human KAP1.
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Positive control
- Purchase matching WB positive control:Recombinant Human KAP1 protein
- Gamma irradiated HeLa cell lysate (WB), and cells (ICC). This antibody gave a positive result in IHC in the following FFPE tissue: Human spleen.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
20C1 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody [20C1] - ChIP Grade (ab22553)
IHC image of KAP1 staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22553, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
