Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5216] to KAP1
Suitable for: WB, IHC-P, ICC/IF
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-KAP1 antibody [EPR5216]
See all KAP1 primary antibodies
Description

Rabbit monoclonal [EPR5216] to KAP1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (Human)
Positive control
- Purchase matching WB positive control:Recombinant Human KAP1 protein
- IHC-P: Human colon or breast carcinoma tissue. WB: HeLa, A431, PC-3, MCF-7 cell lysates. ICC/IF: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR5216
Isotype

IgG
Research areas
- Cancer
- Cell Death
- Apoptosis
- Other
- Epigenetics and Nuclear Signaling
- Bromodomains

Western blot - Anti-KAP1 antibody [EPR5216] (ab109287)

Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Wild type HAP1 + DMSO whole cell lysate (20 µg)
Lane 3: Wild type HAP1 + Blaomycin whole cell lysate (20 µg)
Lane 4: TRIM28 knockout HAP1 whole cell lysate (20 µg)
Lane 5: TRIM28 knockout HAP1 + DMSO whole cell lysate (20 µg)
Lane 6: TRIM28 knockout HAP1 + Blaomycin whole cell lysate (20 µg)
Lane 7: HeLa + DMSO whole cell lysate (20 µg)
Lane 8: HeLa + Blaomycin whole cell lysate (20 µg)

Lanes 1 - 8: Merged signal (red and green). Green - ab109287 observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab109287 was shown to specifically react with KAP1 in wild type cells as signal was lost in KAP1 knockout cells. Wild-type and KAP1 knockout samples were subjected to SDS-PAGE. ab109287 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-KAP1 antibody [EPR5216] (ab109287)

All lanes : Anti-KAP1 antibody [EPR5216] (ab109287) at 1/10000 dilution

Lane 1 : HeLa cell lysates
Lane 2 : A431 cell lysates
Lane 3 : PC-3 cell lysates
Lane 4 : MCF7 cell lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 89 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody [EPR5216] (ab109287)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using 1/250 ab109287.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody [EPR5216] (ab109287)

Immunohistochemical analysis of paraffin-embedded Human colon tissue using 1/250 ab109287.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-KAP1 antibody [EPR5216] (ab109287)

Immunofluorescent staining of HeLa cells using 1/100 ab109287.
Anti-KAP1 antibody [EPR5216] (ab109287)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com