Anti-HP1 gamma/CBX3 antibody (ab10480)
Key features and details
- Rabbit polyclonal to HP1 gamma/CBX3
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HP1 gamma/CBX3 antibody
See all HP1 gamma/CBX3 primary antibodies -
Description
Rabbit polyclonal to HP1 gamma/CBX3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHumanRecombinant fragment
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Immunogen
Synthetic peptide corresponding to Mouse HP1 gamma/CBX3 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab15025) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa; MCF7; HEK293.
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General notes
This product was previously labelled as HP1 gamma
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseHumanRecombinant fragmentApplication Abreviews Notes WB (2) 1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).Notes WB
1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).Target
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Function
Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. -
Sequence similarities
Contains 2 chromo domains. -
Post-translational
modificationsPhosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis. -
Cellular localization
Nucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis. - Information by UniProt
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Database links
- Entrez Gene: 11335 Human
- Entrez Gene: 12417 Mouse
- Omim: 604477 Human
- SwissProt: Q13185 Human
- SwissProt: P23198 Mouse
- Unigene: 381189 Human
- Unigene: 280968 Mouse
- Unigene: 458234 Mouse
see all -
Alternative names
- CBX 3 antibody
- CBX3 antibody
- CBX3_HUMAN antibody
see all
Images
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Western blot - Anti-HP1 gamma/CBX3 antibody (ab10480)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HP1 gamma/CBX3 knockout HAP1 cell lysate (20 µg)
Lane 3: NIH/3T3 cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab10480 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab10480 was shown to recognize HP1 gamma/CBX3 when HP1 gamma/CBX3 knockout samples were used, along with additional cross-reactive bands. Wild-type and HP1 gamma/CBX3 knockout samples were subjected to SDS-PAGE. ab10480 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
Western blot - Anti-HP1 gamma/CBX3 antibody (ab10480)All lanes : Anti-HP1 gamma/CBX3 antibody (ab10480) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Additional bands at: 49 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes -
Western blot - Anti-HP1 gamma/CBX3 antibody (ab10480)Anti-HP1 gamma/CBX3 antibody (ab10480) at 1/500 dilution + Recombinant Human HP1 gamma protein (ab88011) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Exposure time: 30 secondsAb10480 recognizes the tagged recombinant HP1 gamma/CBX3 protein (ab88011) which has an expected molecular weight of ~50 kDa.
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab10480 has been referenced in 19 publications.
- Ma C et al. CBX3 predicts an unfavorable prognosis and promotes tumorigenesis in osteosarcoma. Mol Med Rep 19:4205-4212 (2019). PubMed: 30942427
- Feiner B et al. Risperidone effects on heterochromatin: the role of kinase signaling. Clin Exp Immunol 196:67-75 (2019). PubMed: 30714144
- Sherrard A et al. Streamlined histone-based fluorescence lifetime imaging microscopy (FLIM) for studying chromatin organisation. Biol Open 7:N/A (2018). PubMed: 29535103
- Poulard C et al. Increasing G9a automethylation sensitizes B acute lymphoblastic leukemia cells to glucocorticoid-induced death. Cell Death Dis 9:1038 (2018). PubMed: 30305606
- Chen J et al. The linker histone H1.2 is a novel component of the nucleolar organizer regions. J Biol Chem 293:2358-2369 (2018). PubMed: 29301938
Images
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Western blot - Anti-HP1 gamma/CBX3 antibody (ab10480)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HP1 gamma/CBX3 knockout HAP1 cell lysate (20 µg)
Lane 3: NIH/3T3 cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab10480 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab10480 was shown to recognize HP1 gamma/CBX3 when HP1 gamma/CBX3 knockout samples were used, along with additional cross-reactive bands. Wild-type and HP1 gamma/CBX3 knockout samples were subjected to SDS-PAGE. ab10480 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
Western blot - Anti-HP1 gamma/CBX3 antibody (ab10480)All lanes : Anti-HP1 gamma/CBX3 antibody (ab10480) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Additional bands at: 49 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
-
Western blot - Anti-HP1 gamma/CBX3 antibody (ab10480)Anti-HP1 gamma/CBX3 antibody (ab10480) at 1/500 dilution + Recombinant Human HP1 gamma protein (ab88011) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Exposure time: 30 secondsAb10480 recognizes the tagged recombinant HP1 gamma/CBX3 protein (ab88011) which has an expected molecular weight of ~50 kDa.
