Antibodies, Proteins, Kits and Reagents for Life Science

324 988 ₸ Product size

100 µl 324 988 ₸

Order now and get it on Tuesday March 02, 2021

Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10465(B)] to HP1 gamma/CBX3
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-HP1 gamma/CBX3 antibody [EPR10465(B)]
See all HP1 gamma/CBX3 primary antibodies
Description

Rabbit monoclonal [EPR10465(B)] to HP1 gamma/CBX3
Host species

Rabbit
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human HP1 gamma/CBX3.
Database link: Q13185
Positive control
- WB: NIH/3T3, HeLa, Molt-4 and HepG2 cell lysates, Mouse and Rat brain lysates; IHC-P: Human breast carcinoma tissue, human gastric cancer, Human normal breast tissue.
General notes
This product was previously labelled as HP1 gamma

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10465(B)
Isotype

IgG
Research areas

Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HP1 gamma/CBX3 knockout HAP1 cell lysate (20 µg)
Lane 3: NIH3T3 cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab154871 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab154871was shown to recognize HP1 gamma/CBX3 when HP1 gamma/CBX3 knockout samples were used, along with additional cross-reactive bands. Wild-type and HP1 gamma/CBX3 knockout samples were subjected to SDS-PAGE. ab154871 and ab8245 (loading control to GAPDH) were both diluted to 1/500 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)

All lanes : Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871) at 1/5000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 20 kDa
Observed band size: 21 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric cancer tissue sections labeling HP1 gamma/CBX3 with purified ab154871 at 1:4000 dilution (0.29 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)

All lanes : Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871) at 1/1000 dilution

Lane 1 : NIH/3T3 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Molt-4 cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 20 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling HP1 gamma/CBX3 with ab154871 at 1/50 dilution.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)

Immunohistochemical analysis of paraffin-embedded Human normal breast tissue labeling HP1 gamma/CBX3 with ab154871 at 1/50 dilution.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)

Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154871 showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154871 showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)

Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab154871 showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)

Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab154871 showing +ve staining.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Anti-HP1 gamma/CBX3 antibody [EPR10465(B)] (ab154871)

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