Anti-HP1 gamma/CBX3 antibody (ab227478)
Key features and details
- Rabbit polyclonal to HP1 gamma/CBX3
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HP1 gamma/CBX3 antibody
See all HP1 gamma/CBX3 primary antibodies -
Description
Rabbit polyclonal to HP1 gamma/CBX3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Rhesus monkey
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Immunogen
Recombinant fragment within Human HP1 gamma/CBX3 (internal sequence). The exact sequence is proprietary.
Database link: Q13185 -
Positive control
- WB: HeLa cell extracts; HepG2 whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Mouse duodenum and brain tissues; TOV-21G xenograft tissue. ICC/IF: HeLa cells.
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General notes
This product was previously labelled as HP1 gamma
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HP1 gamma/CBX3 antibody (ab227478)Anti-HP1 gamma/CBX3 antibody (ab227478) at 1/20000 dilution + WT HeLa cell extract at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 21 kDa
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Western blot - Anti-HP1 gamma/CBX3 antibody (ab227478)All lanes : Anti-HP1 gamma/CBX3 antibody (ab227478) at 1/20000 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : HP1 gamma knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 21 kDa15% SDS-PAGE gel.
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Immunoprecipitation - Anti-HP1 gamma/CBX3 antibody (ab227478)HP1 gamma/CBX3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2 µg ab227478. Western blot was performed from the immunoprecipitate using ab227478 at 1/1000 dilution. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HeLa whole cell lysate 40 μg.
Lane 2: Control IP in HeLa whole cell lysate with 2 μg of preimmune rabbit IgG.
Lane 3: ab227478 IP in HeLa whole cell lysate.
15% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody (ab227478)Paraffin-embedded mouse duodenum tissue stained for HP1 gamma/CBX3 using ab227478 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody (ab227478)Paraffin-embedded mouse brain tissue stained for HP1 gamma/CBX3 using ab227478 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-HP1 gamma/CBX3 antibody (ab227478)Anti-HP1 gamma/CBX3 antibody (ab227478) at 1/10000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 21 kDa12% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-HP1 gamma/CBX3 antibody (ab227478)Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HP1 gamma/CBX3 (green) using ab227478 at 1/500 dilution in ICC/IF.
Alpha-tubulin filaments were labeled with an anti-alpha tubulin antibody at 1/2000 dilution (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HP1 gamma/CBX3 antibody (ab227478)Paraffin-embedded TOV-21G (human) xenograft tissue stained for HP1 gamma/CBX3 using ab227478 at 1/250 dilution in immunohistochemical analysis.
