Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10466(B)] to HP1 gamma/CBX3
Suitable for: WB, Flow Cyt
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-HP1 gamma/CBX3 antibody [EPR10466(B)]
See all HP1 gamma/CBX3 primary antibodies
Description

Rabbit monoclonal [EPR10466(B)] to HP1 gamma/CBX3
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HepG2, MOLT4 and HeLa cell lysates; HeLa cells.
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Mouse: In our hands this product works best in WB using cell lines rather than tissue lysates.
This product was previously labelled as HP1 gamma

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
Flow Cyt	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10466(B)
Isotype

IgG
Research areas

Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HP1 gamma/CBX3 knockout HAP1 cell lysate (20 µg)
Lane 3: NIH3T3 cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab154195 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab154195 was shown to recognize HP1 gamma/CBX3 when HP1 gamma/CBX3 knockout samples were used, along with additional cross-reactive bands. Wild-type and HP1 gamma/CBX3 knockout samples were subjected to SDS-PAGE. ab154195 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195)

All lanes : Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195) at 1/1000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : MOLT4 cell lysate
Lane 3 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 20 kDa
Flow Cytometry - Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195)

Flow cytometric analysis of permeabilized HeLa cells labeling HP1 gamma/CBX3 with ab154195 at 1/10 dilution (red) compared with a rabbit IgG negative control antibody (green).
Anti-HP1 gamma/CBX3 antibody [EPR10466(B)] (ab154195)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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