Antibodies, Proteins, Kits and Reagents for Life Science

Anti-KAP1 antibody [BL-248-2G6] (ab243871)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BL-248-2G6] to KAP1
Suitable for: WB, ICC, IHC-P, IP
Reacts with: Mouse, Human

Product name

Anti-KAP1 antibody [BL-248-2G6]
See all KAP1 primary antibodies
Description

Rabbit monoclonal [BL-248-2G6] to KAP1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human KAP1. Surrounding serine 824. NP_005753.1 and Gene ID 10155.
Database link: Q13263
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BL-248-2G6
Isotype

IgG
Research areas
- Cancer
- Cell Death
- Apoptosis
- Other
- Epigenetics and Nuclear Signaling
- Bromodomains

Immunocytochemistry - Anti-KAP1 antibody [BL-248-2G6] (ab243871)

Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling KAP1 using ab243871 at 1/100 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody [BL-248-2G6] (ab243871)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for KAP1 using ab243871 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Immunoprecipitation - Anti-KAP1 antibody [BL-248-2G6] (ab243871)

KAP1 was immunoprecipitated from 1 mg HEK-293T whole cell lysate treated with 100 µM etoposide or untreated, using ab243871 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243871 at 1/1000 dilution.
Lane 1: ab243871 IP in HEK-293T cell lysate treated with 100 µM etoposide.

Lane 2: ab243871 Ip in untreated HEK-293T whole cell lysate.
Lane 3: Control IgG in HEK-293T whole cell lysate treated with 100 µM etoposide.
Detection: Chemiluminescence with an exposure time of 3 seconds
Western blot - Anti-KAP1 antibody [BL-248-2G6] (ab243871)

Western blot analysis using ab243871 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (15 µg).
Lane 2: HEK-293T whole cell lysate (15 µg).
Lane3: MCF7 whole cell lysate (15 µg).
Lane 4: HepG2 whole cell lysate (15 µg).
Lane 5: A549 whole cell lysate (15 µg).
Lane 6: SW620 whole cell lysate (15 µg).
Lane 7: SW620 whole cell lysate (15µg).
SK whole cell lysate (15 µg).
Lane 8: Jurkat whole cell lysate (15 µg).
Lane 9: TCMK-1 whole cell lysate (15 µg).
Lane 10: NIH/3T3 whole cell lysate (15 µg).
Lane 11: CT26 whole cell lysate (15 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds
Anti-KAP1 antibody [BL-248-2G6] (ab243871)

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