Anti-FOXO1A (phospho S256) antibody (ab131339)
Key features and details
- Rabbit polyclonal to FOXO1A (phospho S256)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FOXO1A (phospho S256) antibody
See all FOXO1A primary antibodies -
Description
Rabbit polyclonal to FOXO1A (phospho S256) -
Host species
Rabbit -
Specificity
ab131339 detects FOXO1A only when phosphorylated at serine 256 -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human FOXO1A conjugated to keyhole limpet haemocyanin. Synthetic peptide corresponding to the sequence around phosphorylation site of serine 256 (
A-A-S(p)-M-D
) of Human FOXO1A (NP_002006.2).
Sequence:A-A-S(p)-M-D
Database link: Q12778 -
Positive control
- MCF7 cells, 293 cell extracts, Human breast carcinoma tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.88% Sodium chloride, 49% PBS
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab131339 is purified by affinity-chromatography using an epitode-specific phosphopetide. Non-phoso specific antibodies are removed by chromatography using a non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-FOXO1A (phospho S256) antibody (ab131339)
Immunofluorescent analysis of formalin-fixed, paraffin-embedded human breast tissue with Anti-FOXO1A at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (CY3) at 1/50 was used as secondary.
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Western blot - Anti-FOXO1A (phospho S256) antibody (ab131339)All lanes : Anti-FOXO1A (phospho S256) antibody (ab131339) at 1/500 dilution
Lane 1 : 293 cell extracts
Lane 2 : 293 cell extracts treated with serum
Predicted band size: 69 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-FOXO1A (phospho S256) antibody (ab131339)Immunofluorescence analysis of methanol-fixed MCF7 cells using ab131339 at a 1/100 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A (phospho S256) antibody (ab131339)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab131339 at a 1/50 dilution, in the absence (left) and presence (right) of the blocking peptide.
