Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FOXO1A antibody [EP927Y] - BSA and Azide free (ab219734)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP927Y] to FOXO1A - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB, Flow Cyt (Intra)
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-FOXO1A antibody [EP927Y] - BSA and Azide free
    See all FOXO1A primary antibodies

  • Description

    Rabbit monoclonal [EP927Y] to FOXO1A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WB, Flow Cyt (Intra)more details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • NIH/3T3 cell lysate, human bladder carcinoma tissue and HeLa cells.

  • General notes

    ab219734 is the carrier-free version of ab52857.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXO1A antibody [EP927Y] - BSA and Azide free (ab219734)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXO1A antibody [EP927Y] - BSA and Azide free (ab219734)
    ab52857 at 1/100 dilution staining FOXO1A in HeLa cells by Immunocytochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52857).

  • Flow Cytometry - Anti-FOXO1A antibody [EP927Y] - BSA and Azide free (ab219734)
    Flow Cytometry - Anti-FOXO1A antibody [EP927Y] - BSA and Azide free (ab219734)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling FOXO1A (red) with ab52857 at a 1/90 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52857).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EP927Y] - BSA and Azide free (ab219734)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EP927Y] - BSA and Azide free (ab219734)

    ab52857 at 1/250 dilution staining FOXO1A in human bladder carcinoma by Immunohistochemisty, Paraffin embedded tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52857).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-FOXO1A antibody [EP927Y] - BSA and Azide free (ab219734)
    Anti-FOXO1A antibody [EP927Y] - BSA and Azide free (ab219734)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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