Anti-FOXO1A (phospho S319) antibody (ab47326)
Key features and details
- Rabbit polyclonal to FOXO1A (phospho S319)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FOXO1A (phospho S319) antibody
See all FOXO1A primary antibodies -
Description
Rabbit polyclonal to FOXO1A (phospho S319) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of FOXO1A only when phosphorylated at serine 319.(Human: Ser319; Mouse: Ser316; Rat: Ser313) -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic phosphopeptide derived from human FOXO1A around the phosphorylation site of serine 319 (T-S-SP-N-A)
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Positive control
- human breast carcinoma, HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FOXO1A (phospho S319) antibody (ab47326)All lanes : Anti-FOXO1A (phospho S319) antibody (ab47326)
Lane 1 : extracts from EGF treated HeLa cells
Lane 2 : extracts from HeLa cells
Predicted band size: 70 kDa
Typically 5-30ug of total protein was loaded per lane of the gel. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A (phospho S319) antibody (ab47326)ab47326 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of phospho-peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-FOXO1A (phospho S319) antibody (ab47326)ICC/IF image of ab47326 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47326, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
