Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (ab232590)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12704] to KDM5B / PLU1 / Jarid1B - BSA and Azide free
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free
    See all KDM5B / PLU1 / Jarid1B primary antibodies

  • Description

    Rabbit monoclonal [EPR12704] to KDM5B / PLU1 / Jarid1B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type HAP1 whole cell lysate; HeLa whole cell lysate.

  • General notes

    Ab232590 is the carrier-free version of ab181089. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232590 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (ab232590)
    Immunocytochemistry/ Immunofluorescence - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (ab232590)

    Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling KDM5B / PLU1 / Jarid1B with ab181089 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter-stained with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181089).

  • Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (ab232590)
    Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (ab232590)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: KDM5B knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab181089 observed at 170 kDa. Red - loading control, ab18058, observed at 120 kDa.

    ab181089 was shown to recognize KDM5B in wild-type HAP1 cells as signal was lost at the expected MW in KDM5B knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and KDM5B knockout samples were subjected to SDS-PAGE. ab181089 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1.9 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181089).

  • Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (ab232590)
    Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (ab232590)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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